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Mapping QTLs for tissue culture response in soybean (Glycine max (L.) Merr.)

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Molecules and Cells

Abstract

Quantitative trait loci (QTLs) that control the tissue culture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The molecular map consisting of 834 molecular markers using this population covered space 2307.83 cM of the genome throughout 24 linkage groups. The performance of tissue culture in soybean was evaluated by two indices: callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF). They were expressed as the number of explants producing callus/ the number of total explants and the number of explants producing somatic embryos/ the number of total explants, respectively. The RIL lines showed continuous segregation for both indices. With the composite interval mapping (CIM) described in Windows QTL Cartographer Version 2.5, three quantitative trait loci (QTLs) were identified for the frequency of callus induction, on chromosomes B2 and D2, accounting for phenotypic variation from 5.84% to 16.60%; four QTLs on chromosome G were detected for the frequency of somatic embryos initiation and explained the phenotypic variation from 7.79% to 14.16%. The information of new QTLs identified in the present study will contribute to genetic improvement of regeneration traits with marker-assisted selection (MAS) in soybean.

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Authors and Affiliations

  1. National Center for Soybean Improvement, Nanjing Agricultural University, Weigang No. 1, Nanjing, Jiangsu, 210095, China

    Chao Yang, Tuanjie Zhao, Deyue Yu & Junyi Gai

  2. Department of Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, China

    Chao Yang

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  1. Chao Yang
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  2. Tuanjie Zhao
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  3. Deyue Yu
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  4. Junyi Gai
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Correspondence to Junyi Gai.

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Yang, C., Zhao, T., Yu, D. et al. Mapping QTLs for tissue culture response in soybean (Glycine max (L.) Merr.). Mol Cells 32, 337–342 (2011). https://doi.org/10.1007/s10059-011-0063-1

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  • DOI: https://doi.org/10.1007/s10059-011-0063-1

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