Abstract.
Quantitative trait loci (QTLs) controlling callus growth (CG), subsequent shoot differentiation ratio (SD) and green shoot ratio (GS) in immature embryo culture were identified in barley. A base map was developed from 99 recombinant inbred lines (RILs) of 'Azumamugi' × 'Kanto Nakate Gold'. The tissue-culture traits were evaluated at the F7 and F10 generations of the RILs. The RILs showed wide and continuous variations in each of the three tissue-culture traits. Three QTLs for CG, three QTLs for SD and two QTLs for GS were detected by using composite interval mapping. A QTL for SD on chromosome 3H had a large effect, and 'Kanto Nakate Gold', which has a high differentiation ability, contributed to this QTL. The location of this QTL is identical to, or very close to, the uzu locus. We discuss the relationships between tissue-culture loci in 'Azumamugi' × 'Kanto Nakate Gold' and those in other mapping populations.
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Mano, .Y., Komatsuda, .T. Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.). Theor Appl Genet 105, 708–715 (2002). https://doi.org/10.1007/s00122-002-0992-3
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DOI: https://doi.org/10.1007/s00122-002-0992-3