AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower (Helianthus annuus L.)

Abstract 

Genetic control for two in vitro organogenesis traits, the number of shoots per explant plated (S/E) and the number of shoots per regenerating explant (S/RE), was investigated in 75 recombinant inbred lines (RILs) of sunflower and their two parents (PAC-2 and RHA-266). Genetic variability was observed among the 75 RILs for the organogenesis traits studied. Some RILs presented significant differences when compared with the best parental line (RHA-266). Genetic gain, in terms of the percentage of the best parent, for 32% of the selected RILs was significant. A set of 99RILs from the same cross including the 75 mentioned above was screened with 333 AFLP markers and a linkage map was constructed based on 264 linked loci. Six putative QTLs for the S/RE (tentatively named osr) and seven QTLs for the S/E (ose) trait were detected using composite interval mapping. For each trait, the QTLs explained 52% (ose) and 67% (osr) of the total phenotypic variance. These results suggested that additive gene effects predominate in explaining a large proportion of the observed genetic variation associated with regeneration ability. The coincidental location of QTLs for S/E and S/RE is discussed.