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Evaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India

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Abstract

Symplocos laurina Wall., an important medicinal tree species, is one of the pioneering plants for establishment of shola forest ecosystem. We analyzed 208 accessions of S. laurina from 13 populations belonging to three ecoregions in India namely, Western Ghats, Eastern Ghats and Northeast India, to comment on the genetic diversity and population structure of the species. Of the 100 inter simple sequence repeat primers screened, eight were selected based on their clear and reproducible polymorphic patterns. These eight ISSR primers produced on an average 1,014 bands from 208 accessions, of which 6.58 % were rare bands, 55.96 % were shared bands and 37.46 % were similar bands. The primers, UBC835 and UBC880, amplified the highest number of polymorphic loci (16), while the primer UBC852 amplified the least number of amplicons (9). The values for total genetic diversity (H T) and the average heterozygosity (H S) were 0.3407 and 0.2263, respectively; while the coefficient of genetic differentiation (G ST) was 0.3358. Within-population variance was higher (68 %) than among-population variance (17 %), indicating high intra-population genetic diversity. These populations were grouped according to their geographical location in cluster analysis. Mantel test also showed significant positive correlation between genetic and geographical distances. The present study revealed the importance of the Palghat gap in South India in shaping the distribution of genetic diversity in S. laurina.

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Acknowledgments

The authors thank Forest Departments from the states of Maharashtra, Meghalaya, Karnataka, Kerala and Tamil Nadu for permitting sample collection from the Western Ghats, Eastern Ghats and Northeast India and the staff at various field stations for their help and co-operation. The authors also thank Drs. RS Kulkarni (Indian Institute of Science Education and Research, Pune), BG Kulkarni, PSN Rao (Botanical Survey of India, Western Circle, Pune) and HS Suresh (Center for Ecological Science, Indian Institute of Science, Bangalore) for their help during sample collections. SB is thankful to the Council for Scientific and Industrial Research (CSIR) for senior research fellowship. This work was financially supported by the Department of Biotechnology, New Delhi to CSIR-NCL, Pune and BSI, Pune, India.

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  1. Sofia Banu

    Present address: Department of Bioengineering and Technology, Gauhati University, Guwahati, 781014, India

Authors and Affiliations

  1. Plant Molecular Biology Group, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, 411008, India

    Rasika M. Bhagwat, Sofia Banu, Bhushan B. Dholakia, Narendra Y. Kadoo, Meena D. Lagu & Vidya S. Gupta

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  1. Rasika M. Bhagwat
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Correspondence to Vidya S. Gupta.

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Bhagwat, R.M., Banu, S., Dholakia, B.B. et al. Evaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India. Plant Syst Evol 300, 2239–2247 (2014). https://doi.org/10.1007/s00606-014-1046-4

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