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Development of a genetic linkage map for Pinus radiata and detection of pitch canker disease resistance associated QTLs

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The heritability of genetic resistance of radiata pine against Fusarium circinatum was not clear. We demonstrated that there are at least 3 QTLs that could be involved in this resistance/susceptibility.

Abstract

A genetic linkage map was developed for Pinus radiata, using Amplified Fragment Length Polymorphism (AFLP), Inter-Simple Sequence Repeat (ISSR), Selective Amplification of Microsatellite Polymorphic Loci (SAMPL), and Simple Sequence Repeat (SSR) molecular markers, based on a two-way pseudo-testcross strategy, using 86 individuals of a F1 full-sib family and 787 molecular markers for genotyping. Linkage analysis generated a map of medium to high density for each parent, with 1,060 and 1,258 cM for parents XO and XP, respectively. A total of 458 markers were mapped on 12 linkage groups (LG) in XO and XP, which equals the number of haploid chromosomes present in P. radiata. Analysis of quantitative trait loci (QTL) for resistance against pitch canker disease caused by Fusarium circinatum was made using Bayesian Information Criterion (BIC). In the XO parental map, two groups (LG-1 and LG-9) showed high probabilities for one or more QTLs. Only one group (LG-9) in the XP parental map showed probability for one or more QTLs. The results indicate that resistance to pitch canker is inherited from both parents. These results provide the basis for further studies focused on structure, evolution, and function of the P. radiata genome.

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Acknowledgments

This work was financed by Genómica Forestal (CORFO, grant number 05CTE04-02) and a PhD grant from Comisión Nacional de Ciencia y Tecnología (23100216) to PM.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Centro de Biotecnología y Facultad de Ciencias Forestales Casilla 160-C, Universidad de Concepción, Concepción, Chile

    P. Moraga-Suazo, L. Orellana, P. Quiroga, E. Sanfuentes & S. Valenzuela

  2. BIOFOREST, Camino a Coronel, Km. 15, Concepción, Chile

    C. Balocchi

  3. Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA

    R. W. Whetten

  4. Genómica Forestal S.A., Concepción, Chile

    R. Hasbún

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  1. P. Moraga-Suazo
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  2. L. Orellana
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  3. P. Quiroga
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  4. C. Balocchi
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  5. E. Sanfuentes
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  6. R. W. Whetten
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  7. R. Hasbún
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  8. S. Valenzuela
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Correspondence to S. Valenzuela.

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Communicated by J. Carlson.

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Moraga-Suazo, P., Orellana, L., Quiroga, P. et al. Development of a genetic linkage map for Pinus radiata and detection of pitch canker disease resistance associated QTLs. Trees 28, 1823–1835 (2014). https://doi.org/10.1007/s00468-014-1090-2

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