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Genetic dissection of fusiform rust and pitch canker disease traits in loblolly pine

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Abstract

Loblolly pine (Pinus taeda L.) exhibits genetic resistance to fusiform rust disease (incited by the biotrophic fungus, Cronartium quercuum f. sp. fusiforme) and pitch canker disease (incited by the necrotrophic fungus, Fusarium circinatum). In this study, a total of 14,015 loblolly pine cuttings from 1,065 clones were screened in controlled greenhouse conditions to identify phenotypes of clones, families, and parents that guide a genetic dissection of disease traits associated with pitch canker and fusiform rust. A total of 23,373 phenotypic data points were collected for lesion length (pitch canker) and gall score, gall length, and gall width (fusiform rust). We verified heritable fusiform rust and pitch canker traits and calculated parental, clonal, and full-sib family rankings for both diseases. Genetic correlations revealed that traits associated with fusiform rust are genetically distinct from one another, and that the genetic mechanisms underlying pitch canker and fusiform rust resistance are independent. The disease phenotyping described here is a critical step towards identifying specific loci and alleles associated with fusiform rust and pitch canker resistance.

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Acknowledgements

This is journal article number R-10656 of the Florida Agricultural Experiment Station. The work was supported by USDA–CSREES–IFAFS (grant no. 2001-52100-11315) with in-kind contributions from the Forest Biology Research Cooperative at the University of Florida. We thank Carol Young, Katherine Smith, and Chris Dervinis for technical assistance, and Henry Amerson and Robert Schmidt for providing inoculum and helpful discussions. We gratefully acknowledge the support of many individuals for assistance with phenotyping at the Resistance Screening Center and UF. These people include the FBRC staff, especially Brian Roth, Brian Baltunis and Emilio Ancaya, staff from Sarah Covert’s lab group, Keng Yen Lee, and members of the Davis lab group.

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Authors and Affiliations

  1. School of Forest Resources and Conservation, University of Florida, PO Box 110410, Gainesville, FL, 32611, USA

    Gogce C. Kayihan, Dudley A. Huber, Alison M. Morse, Timothy L. White & John M. Davis

  2. Program in Plant Molecular and Cellular Biology, University of Florida, PO Box 110410, Gainesville, FL, 32611, USA

    John M. Davis & John M. Davis

Authors
  1. Gogce C. Kayihan
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  2. Dudley A. Huber
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  3. Alison M. Morse
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  4. Timothy L. White
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  5. John M. Davis
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Correspondence to John M. Davis.

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Communicated by D.B. Neale

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Kayihan, G.C., Huber, D.A., Morse, A.M. et al. Genetic dissection of fusiform rust and pitch canker disease traits in loblolly pine. Theor Appl Genet 110, 948–958 (2005). https://doi.org/10.1007/s00122-004-1915-2

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  • DOI: https://doi.org/10.1007/s00122-004-1915-2

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