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QTL associations for density and diameter in Pinus radiata and the potential for marker-aided selection

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Abstract

A large full-sib family of radiata pine (Pinus radiata Donn. ex D. Don) was used for quantitative trait locus (QTL) detection and independent verification. QTL detection experiments were carried out for juvenile wood density (JWD) and stem diameter at breast height (DBH) using selective genotyping. Evenly spaced RFLP and microsatellite markers were selected from an existing linkage map. QTLs were verified in an independent set of progeny from the same family. Based on map location, at least eight QTL positions for JWD and two for DBH were detected and verified. The percent variance accounted for by the markers ranged from 0.78% to 3.58%, suggesting a genomic architecture of many genes with small effect. Two unrelated “bridging” families were chosen as candidates for marker-aided selection (MAS), and six microsatellite markers showing an association with JWD or DBH were tested in these families. Of these, four markers showed a consistent association with JWD in one or both of the bridging families. Results from this study provide a basis for MAS in P. radiata.

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Acknowledgements

The QTL detection project reported here continues work initiated as part of the GEENZ Ltd program when S.D.C. was employed by Forest Research. Most of the work for this project was carried out while S.D.C. was employed by Trees & Technology Ltd. We acknowledge Philip Wilcox and John Lee at Forest Research for their contribution to collection and analyses of the wood density data, and David Darling (Rubicon) for his support of the project. Thanks to Merv Shepherd and Mike Cross at Southern Cross University for help with DNA extractions, and Gavin Moran and Harry Wu at CSIRO for helpful reviews of the manuscript.

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Authors and Affiliations

  1. CSIRO Forestry and Forest Products, ACT 2604, Canberra, Australia

    M. E. Devey, M. F. Nolan & A. C. Matheson

  2. Carson Associates Ltd., Rotorua, New Zealand

    S. D. Carson

  3. Trees & Technology Ltd., Te Teko, New Zealand

    C. Te Riini & J. Hohepa

Authors
  1. M. E. Devey
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  2. S. D. Carson
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  3. M. F. Nolan
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  4. A. C. Matheson
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  5. C. Te Riini
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  6. J. Hohepa
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Corresponding author

Correspondence to M. E. Devey.

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Communicated by D.B. Neale

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Devey, M.E., Carson, S.D., Nolan, M.F. et al. QTL associations for density and diameter in Pinus radiata and the potential for marker-aided selection. Theor Appl Genet 108, 516–524 (2004). https://doi.org/10.1007/s00122-003-1446-2

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  • DOI: https://doi.org/10.1007/s00122-003-1446-2

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