Abstract
Six related radiata pine (Pinus radiata) full-sib families were used to detect and independently verify quantitative trait loci (QTLs) for resistance to Dothistroma needle blight, caused by Dothistroma septospora. The detection families had from 26 to 30 individuals each, and had either a common maternal (31053) or paternal (31032) parent; one family (cross 4) consisted of progeny from both parents, 31053×31032. Approximately 200 additional progeny from cross 4 were clonally replicated and planted at two sites, with at least five to seven ramets of each individual per site. Marker segregation data were collected from a total of 250 RFLP and microsatellite markers, and single factor ANOVAs were conducted separately for each family and marker. A number of putative associations were observed, some across more than one family. Permutation tests were used to confirm expected probabilities of multiple associations based on chance alone. Seven markers representing at least four QTLs for resistance to Dothistroma were identified as being significant in more than one family; one of these was significant at P<0.05 in three families and highly significant at P<0.01 in a fourth. Further confirmation was obtained by testing those markers that were significant in more than one of the detection families (or highly significant in cross 4) in the clonally replicated progeny from cross 4. Four QTL positions were verified in the clonal populations, with a total percent variation accounted for of 12.5.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ades PK, Simpson JA (1990) Clonal selection for resistance to Dothistroma needle blight in Pinus radiata. New Forests 4:27–35
Beavis WD (1998) QTL analysis: power, precision and accuracy. In: Paterson AH (ed) Molecular dissection of complex traits. CRC Press, Boca Raton, pp 145–162.
Bradshaw HD, Forster GS (1992) Marker-aided selection and propagation systems in trees: advantages of cloning for studying quantitative inheritance. Can J For Res 22:1044–1049
Bradshaw HD, Stettler RF (1995) Molecular genetics of growth and development in Populus.: IV. Mapping QTLs with large effects on growth, form and phenology traits in a forest tree. Genetics 139:963–973
Byrne M, Murrell JC, Owen JV, Kriedemann P, Williams ER, Moran GF (1997a) Identification and mode of action of quantitative trait loci affecting seedling height and leaf area in Eucalyptus nitens. Theor Appl Genet 94:674–681
Byrne M, Murrell JC, Owen JV, Williams ER, Moran GF (1997b) Mapping of quantitative trait loci influencing frost tolerance in Eucalyptus nitens. Theor Appl Genet 95:975–979
Carson SD (1989) Selecting Pinus radiata for resistance to Dothistroma needle blight. NZ J For Sci 19:3–21
Carson SD, Djorovic N, Djorovic A, Wilcox P, Ball R (2003a) Simulation of QTL detection and MAS for quantitative traits. I. Impact of population size, underlying genetic structure, and methods of choosing markers. Genetics (in press)
Carson SD, Djorovic N, Djorovic A, Wilcox P, Carson M, Ball R (2003b) Simulation of QTL detection and MAS for quantitative traits II: Comparison of gain and selection bias for alternate experimental designs including selective genotyping and map density. Genetics (in press)
Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971
Devey ME, Bell JC, Smith DN, Neale DB, Moran GF (1996) A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers. Theor Appl Genet 92:673–679
Devey ME, Sewell MM, Uren TL, Neale DB (1999) Comparative mapping in loblolly pine using RFLP and microsatellite markers. Theor Appl Genet 99:656–662
Devey ME, Bell JC, Uren TL, Moran GF (2002) A set of microsatellite markers for fingerprinting and breeding applications in Pinus radiata. Genome 45:984–989
Devey ME, Carson SD, Nolan MF, Matheson AC, Te Riini A, Hohepa J (2003) QTL associations for density and diameter in Pinus radiata and the potential for marker-aided selection. Theor Appl Genet (accepted)
Emebiri LC, Devey ME, Matheson AC, Slee MU (1997) Linkage of RAPD markers to NESTUR, a stem growth index in radiata pine seedlings. Theor Appl Genet 95:119–124
Grattapaglia D, Bertolucci FL, Sederoff RR (1995) Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross strategy and RAPD markers. Theor Appl Genet 90:933–947
Groover A, Devey M, Fiddler T, Lee J, Megraw R, Mitchel-Olds T, Sherman B, Vujcic S, Williams C, Neale D (1994) Identification of quantitative trait loci influencing wood specific gravity in an outbred pedigree of loblolly pine. Genetics 138:1293–1300
Jermstad KD, Bassoni DL, Jech KS, Wheeler NC, Neale DB (2001) Mapping of quantitative trait loci controlling adaptive traits in coastal Douglas-fir. I. Timing of vegetative bud flush. Theor Appl Genet 102:1142–1151
Kumar S, Carson SD, Garrick DJ (2000) Detecting linkage between a fully-informative marker locus and a trait locus in outbred populations using analysis of variance. For Genet 7:47–56
Melchinger AE, Utz HF, Schön CC (1998) Quantitative trait locus (QTL) mapping in different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects. Genetics 149:383–403
Muranty, H 1996. Power of tests for quantitative trait loci detection using full-sib families in different schemes. Heredity 76:156–165
Old KM, Dudzinski MJ (1999) Forest pathogen introductions to Australia: experiences, threats and counter measures. In: Devey ME, Matheson AC, Gordon TR (eds) Current and potential impacts of pitch canker in radiata pine. Proc IMPACT Monterey Workshop. CSIRO, Australia, pp 11–20
Pas JB van der(1981) Reduced early growth rates of Pinus radiata caused by Dothistroma pini. NZ J For Sci 11:210–20
Sewell MM, Neale DB (2000) Mapping quantitative traits in forest trees. In: Jain SM, Minocha SC (eds) Molecular biology of woody plants, forestry sciences, vol 64. Kluwer, Dordrecht, pp 407–423
Sewell MM, Bassoni DL, Megraw RA, Wheeler NC, Neale DB (2000) Identification of QTLs influencing wood property traits in loblolly pine (Pinus taeda L.). I. Physical wood properties. Theor Appl Genet 101:1273–1281
Woollons RC, Hayward WJ (1984) Growth losses in Pinus radiata unsprayed for Dothistroma pini. NZ J For Sci 14:14–22
Acknowledgements
Funding and in-kind support for this research was provided by STBA, State Forests of NSW, and the Forest and Wood Products Research and Development Corporation.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by D.B. Neale
Rights and permissions
About this article
Cite this article
Devey, M.E., Groom, K.A., Nolan, M.F. et al. Detection and verification of quantitative trait loci for resistance to Dothistroma needle blight in Pinus radiata . Theor Appl Genet 108, 1056–1063 (2004). https://doi.org/10.1007/s00122-003-1471-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-003-1471-1