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Releasing intracellular product to prepare whole cell biocatalyst for biosynthesis of Monascus pigments in water–edible oil two-phase system

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Abstract

Selective releasing intracellular product in Triton X-100 micelle aqueous solution to prepare whole cell biocatalyst is a novel strategy for biosynthesis of Monascus pigments, in which cell suspension culture exhibits some advantages comparing with the corresponding growing cell submerged culture. In the present work, the nonionic surfactant Triton X-100 was successfully replaced by edible plant oils for releasing intracellular Monascus pigments. High concentration of Monascus pigments (with absorbance nearly 710 AU at 470 nm in the oil phase, normalized to the aqueous phase volume approximately 142 AU) was achieved by cell suspension culture in peanut oil–water two-phase system. Furthermore, the utilization of edible oil as extractant also fulfills the demand for application of Monascus pigments as natural food colorant.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (No: 21276155).

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Authors and Affiliations

  1. School of Pharmacy, State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, China

    Minglue Hu & Zhilong Wang

  2. School of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China

    Xuehong Zhang

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  1. Minglue Hu
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  2. Xuehong Zhang
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  3. Zhilong Wang
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Correspondence to Zhilong Wang.

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Hu, M., Zhang, X. & Wang, Z. Releasing intracellular product to prepare whole cell biocatalyst for biosynthesis of Monascus pigments in water–edible oil two-phase system. Bioprocess Biosyst Eng 39, 1785–1791 (2016). https://doi.org/10.1007/s00449-016-1654-1

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