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Biological synthesis of Au nanoparticles using liquefied mash of cassava starch and their functionalization for enhanced hydrolysis of xylan by recombinant xylanase

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Abstract

Au nanoparticles (AuNPs) have shown the potential for a variety of applications due to their unique physical and chemical properties. In this study, a facile and affordable method for the synthesis of AuNPs via the liquefied mash of cassava starch has been described and the functionalized AuNPs by l-cysteine improved activity of recombinant xylanase was demonstrated. UV–Vis absorption spectroscopy, transmission electron microscopy, and zeta potential measurements were performed to characterize the AuNPs and monitor their synthesis. The presence of Au was confirmed by energy-dispersive X-ray spectroscopy (EDX) and the X-ray diffraction patterns showed that Au nanocrystals were face-centered cubic. The C=O stretching vibration in the Fourier transform infrared spectrum of AuNPs suggested that the hemiacetal C–OH of sugar molecules performed the reduction of Au3+ to Au0. The presence of C and O in the EDX spectrum and the negative zeta potential of AuNPs suggested that the biomolecules present in liquefied cassava mash were responsible for the stabilization of AuNPs. The surface of AuNPs was easily functionalized by l-cysteine, which improved the stability of AuNPs. Moreover, cysteine-functionalized AuNPs could significantly improve recombinant xylanase efficiency and stability.

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Acknowledgments

The authors thank Qi Yang for providing the recombinant xylanase rAfxynA. This investigation was supported by grants from the Guangxi Bagui Scholars Program Foundation (Grant Number 2011A001) and the Guangxi Natural Science Foundation (Grant Numbers 2012GXNSFGA060005 and 2013GXNSFBA019098).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, People’s Republic of China

    Sumei Zeng, Liangwei Du, Meiying Huang & Jia-Xun Feng

  2. College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, People’s Republic of China

    Sumei Zeng & Jia-Xun Feng

  3. College of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, People’s Republic of China

    Liangwei Du & Meiying Huang

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  1. Sumei Zeng
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  2. Liangwei Du
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  4. Jia-Xun Feng
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Correspondence to Jia-Xun Feng.

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Zeng, S., Du, L., Huang, M. et al. Biological synthesis of Au nanoparticles using liquefied mash of cassava starch and their functionalization for enhanced hydrolysis of xylan by recombinant xylanase. Bioprocess Biosyst Eng 39, 785–792 (2016). https://doi.org/10.1007/s00449-016-1558-0

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  • DOI: https://doi.org/10.1007/s00449-016-1558-0

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