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Rapid extra-/intracellular biosynthesis of gold nanoparticles by the fungus Penicillium sp.

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Abstract

In this work, the fungus Penicillium was used for rapid extra-/intracellular biosynthesis of gold nanoparticles. AuCl4 − ions reacted with the cell filtrate of Penicillium sp. resulting in extracellular biosynthesis of gold nanoparticles within 1 min. Intracellular biosynthesis of gold nanoparticles was obtained by incubating AuCl4 − solution with fungal biomass for 8 h. The gold nanoparticles were characterized by means of visual observation, UV–Vis absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The extracellular nanoparticles exhibited maximum absorbance at 545 nm in UV–Vis spectroscopy. The XRD spectrum showed Bragg reflections corresponding to the gold nanocrystals. TEM exhibited the formed spherical gold nanoparticles in the size range from 30 to 50 nm with an average size of 45 nm. SEM and TEM revealed that the intracellular gold nanoparticles were well dispersed on the cell wall and within the cell, and they are mostly spherical in shape with an average diameter of 50 nm. The presence of gold was confirmed by EDX analysis.

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References

  • Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M (2003a) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B Biointerfaces 28:313–318

    Article  CAS  Google Scholar 

  • Ahmad A, Senapati S, Khan MI, Kumar R, Sastry M (2003b) Extracellular biosynthesis of monodisperse gold nanoparticles by a novel extremophilic actinomycete, Thermomonospora sp. Langmuir 19:3550–3553. doi:10.1021/la026772l

    Article  CAS  Google Scholar 

  • Ahmad A, Senapati S, Khan MI, Kumar R, Sastry M (2005) Extra-/intracellular biosynthesis of gold nanoparticles by an alkalotolerant fungus, Trichothecium sp. J Biomed Nanotech 1:47–53. doi:10.1166/jbn.2005.012

    Article  CAS  Google Scholar 

  • Anshup, Venkataramen JS, Subramaniam C, Kumar RR, Priya S, Kumar TRS, Omkumar RV, John A, Pradeep T (2005) Growth of gold nanoparticles in human cells. Langmuir 21:11562–11567. doi:10.1021/la0519249

    Article  CAS  Google Scholar 

  • Balaji DS, Basavaraja S, Deshpande R, Mahesh DB, Prabhakar BK, Venkataraman A (2009) Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Colloids Surf B Biointerfaces 68:88–92. doi:10.1016/j.colsurfb.2008.09.022

    Article  CAS  Google Scholar 

  • Bhainsa KC, D’Souza SF (2006) Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf B Biointerfaces 47:160–164. doi:10.1016/j.colsurfb.2005.11.026

    Article  CAS  Google Scholar 

  • Birla SS, Tiwari VV, Gade AK, Ingle AP, Yadav AP, Rai MK (2009) Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Lett Appl Microbiol 48:173–179. doi:10.1111/j.1472-765X.2008.02510.x

    Article  CAS  Google Scholar 

  • Deplanche K, Macaskie LE (2008) Biorecovery of gold by Escherichia coli and Desulfovibrio desulfuricans. Biotechnol Bioeng 99:1055–1064. doi:10.1002/bit.21688

    Article  CAS  Google Scholar 

  • Dickson DPE (1999) Nanostructured magnetism in living systems. J Magn Magn Mater 203:46–49

    Article  CAS  Google Scholar 

  • Du LW, Hong J, Liu XH, Wang EK (2007) Biosynthesis of gold nanoparticles assisted by Escherichia coli DH5α and its application on direct electrochemistry of hemoglobin. Electrochem Commun 9:1165–1170. doi:10.1016/j.elecom.2007.01.007

    Article  CAS  Google Scholar 

  • Durán N, Marcato PD, Alves OL, Souza GI, Esposito E (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotech 3:8–14. doi:10.1186/1477-3155-3-8

    Article  Google Scholar 

  • Fayaz AM, Balaji K, Kalaichelvan PT, Venkatesan R (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine NBM 6:103–109. doi:10.1016/j.nano.2009.04.006

    CAS  Google Scholar 

  • Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M (2009) Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine NBM 5:382–386. doi:10.1016/j.nano.2009.06.005

    CAS  Google Scholar 

  • Ganesh BMM, Gunasekaran P (2009) Production and structural characterization of crystalline silver nanoparticles from Bacillus cereus isolate. Colloids Surf B Biointerfaces 74:191–195. doi:10.1016/j.colsurfb.2009.07.016

    Article  Google Scholar 

  • Gurunathan S, Kalishwaralal K, Vaidyanathan R, Deepak V, Pandian SRK, Muniyandi J, Hariharan N, Eom SH (2009) Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Colloids Surf B Biointerfaces 74:328–335. doi:10.1016/j.colsurfb.2009.07.048

    Article  CAS  Google Scholar 

  • He SY, Guo ZR, Zhang Y, Zhang S, Wang J, Gu N (2007) Biosynthesis of gold nanoparticles using the bacteria Rhodopseudomonas capsulata. Mater Lett 61:3984–3987. doi:10.1016/j.matlet.2007.01.018

    Article  CAS  Google Scholar 

  • He SY, Zhang Y, Gu ZR, Gu N (2008) Biological synthesis of gold nanowires using extract of Rhodopseudomonas capsulata. Biotechnol Prog 24:476–480. doi:10.1021/bp0703174

    Article  CAS  Google Scholar 

  • Husseiny MI, El-Aziz MA, Badr Y, Mahmoud MA (2007) Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spectrochimica Acta Part A 67:1003–1006. doi:10.1016/j.saa.2006.09.028

    Article  CAS  Google Scholar 

  • Ingle A, Rai M, Gade A, Bawaskar M (2009) Fusarium solani: a novel biological agent for the extracellular synthesis of silver nanoparticles. J Nanopart Res 11:2079–2085. doi:10.1007/s11051-008-9573-y

    Article  CAS  Google Scholar 

  • Joerger TK, Joerger R, Olsson E, Granqvist CG (2001) Bacteria as workers in the living factory: metal-accumulating bacteria and their potential for materials science. Trends Biotechnol 19:15–20

    Article  Google Scholar 

  • Kajander EO (2006) Nanobacteria-propagating calcifying nanoparticles. Lett Appl Microbiol 42:549–552. doi:10.1111/j.1472-765X.2006.01945.x

    CAS  Google Scholar 

  • Kalimuthu K, Babu RS, Venkataraman D, Bilal M, Gurunathan S (2008) Biosynthesis of silver nanocrystals by Bacillus licheniformis. Coll Surf B Biointerfaces 65:150–153. doi:10.1016/j.colsurfb.2008.02.018

    Article  CAS  Google Scholar 

  • Kalimuthu K, Deepak V, Pandian SRK, Gurunathan S (2009) Biological synthesis of gold nanocubes from Bacillus licheniformis. Bioresour Technol 100:5356–5358. doi:10.1016/j.biortech.2009.05.051

    Article  Google Scholar 

  • Kalishwaralal K, Deepak V, Ramkumarpandian S, Nellaiah H, Sangiliyandi G (2008) Extracellular biosynthesis of silver nanoparticles by the culture supernatant of Bacillus licheniformis. Mater Lett 62:4411–4413. doi:10.1016/j.matlet.2008.06.051

    Article  CAS  Google Scholar 

  • Kathiresan K, Manivannan S, Nabeel MA, Dhivya B (2009) Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids Surf B Biointerfaces 71:133–137. doi:10.1016/j.colsurfb.2009.01.016

    Article  CAS  Google Scholar 

  • Kowshik M, Ashtaputre S, Kharrazi S, Vogel W, Urban J, Kulkarni SK, Paknikar KM (2003) Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3. Nanotechnology 14:95–100

    Article  CAS  Google Scholar 

  • Kumar SA, Abyaneh MK, Gosavi SW, Kulkarni SK, Pasricha R, Ahmad A, Khan MI (2007) Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3. Biotechnol Lett 29:439–445. doi:10.1007/s10529-006-9256-7

    Article  CAS  Google Scholar 

  • Lee WP, Chen HS, Dryfe R, Ding YL (2009) Kinetics of nanoparticle synthesis by liquid-liquid interfacial reaction. Coll Surf A Physicochem Eng Asp 343:3–7. doi:10.1016/j.colsurfa.2009.01.040

    Article  CAS  Google Scholar 

  • Leff DV, Brandt L, Heath JR (1996) Synthesis and characterization of hydrophobic, organically soluble gold nanocrystals functionalized with primary amines. Langmuir 12:4723–4730. doi:10.1021/la960445u

    Article  CAS  Google Scholar 

  • Link S, El-Sayed (1999) Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J Phys Chem B 103:8410–8426. doi:10.1021/jp9917648

    Article  CAS  Google Scholar 

  • Link S, El-Sayed (2000) Shape and size dependence of radiative, non-radiative and photothermal properties of gold nanocrystals. Int Rev Phys Chem 19:409–453

    Article  CAS  Google Scholar 

  • Losic D, Mitchell JG, Voelcker NH (2005) Complex gold nanostructures derived by templating from diatom frustules. Chem Commun 39:4905–4907. doi:10.1039/b508733c

    Article  Google Scholar 

  • Mandal D, Bolander ME, Mukhopadhyay D, Sarkar G, Mukherjee P (2006) The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbiol Biotechnol 69:485–492. doi:10.1007/s00253-005-0179-3

    Article  CAS  Google Scholar 

  • Mohanpuria P, Rana NK, Yadav SK (2008) Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 10:507–517. doi:10.1007/s11051-007-9275-x

    Article  CAS  Google Scholar 

  • Mokhtari N, Daneshpajouh S, Seyedbagheri S, Atashdehghan R, Abdi K, Sarkar S, Minaian S, Shahverdi HR, Shahverdi AR (2009) Biological synthesis of very small silver nanoparticles by culture supernatant of Klebsiella pneumonia: The effects of visible-light irradiation and the liquid mixing process. Mater Res Bull 44:1415–1421. doi:10.1016/j.materresbull.2008.11.021

    Article  CAS  Google Scholar 

  • Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Ramani R, Parischa R, Ajayakumar PV, Alam M, Sastry M, Kumar R (2001a) Bioreduction of AuCl -4 ions by the fungus, Verticillium sp. and surface trapping of the gold nanoparticles formed. Angew Chem Int Ed 40:3585–3588

    Article  CAS  Google Scholar 

  • Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Parishcha R, Ajaykumar PV, Alam M, Kumar R, Sastry M (2001b) Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis. Nano Lett 1:515–519. doi:10.1021/nl0155274

    Article  CAS  Google Scholar 

  • Mukherjee P, Senapati S, Mandal D, Ahmad A, Khan MI, Kumar R, Sastry M (2002) Extracellular synthesis of gold nanoparticles by the fungus Fusarium oxysporum. ChemBioChem 3:461–463

    Article  CAS  Google Scholar 

  • Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12:788–800. doi:10.1021/la9502711

    Article  CAS  Google Scholar 

  • Nair B, Pradeep T (2002) Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains. Crys Grow Des 2:293–298. doi:10.1021/cg0255164

    Article  CAS  Google Scholar 

  • Nanda A, Saravanan M (2009) Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE. Nanomedicine NBM 5:452–456. doi:10.1016/j.nano.2009.01.012

    CAS  Google Scholar 

  • Nangia Y, Wangoo N, Goyal N, Shekhawat G, Suri CR (2009) A novel bacterial isolate Stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticles. Microb Cell Fact 8:39. doi:10.1186/1475-2859-8-39

    Article  Google Scholar 

  • Sadowski Z, Maliszewska IH, Grochowalska B, Polowczyk I, Koźlecki T (2008) Synthesis of silver nanoparticles using microorganisms. Mater Sci Pol 26:419–424

    CAS  Google Scholar 

  • Samadi N, Golkaran D, Eslamifar A, Jamalifar H, Fazeli MR, Mohseni FA (2009) Intra/extracellular biosynthesis of silver nanoparticles by an autochthonous strain of Proteus mirabilis isolated from photographic waste. J Biomed Nanotechnol 5:247–253

    Article  CAS  Google Scholar 

  • Sanghi R, Verma P (2009) Biomimetic synthesis and characterisation of protein capped silver nanoparticles. Bioresour Technol 100:501–504. doi:10.1016/j.biortech.2008.05.048

    Article  CAS  Google Scholar 

  • Sastry M, Ahmad A, Khan MI, Kumar R (2003) Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci 85:162–170

    CAS  Google Scholar 

  • Shahverdi AR, Minaeian S, Shahverdi HR, Jamalifar H, Nohi AA (2007) Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Proc Biochem 42:919–923. doi:10.1016/j.procbio.2007.02.005

    Article  CAS  Google Scholar 

  • Shankar SS, Ahmad A, Pasricha R, Sastry M (2003) Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J Mater Chem 13:1822–1826. doi:10.1039/b303808b

    Article  CAS  Google Scholar 

  • Tseng KH, Huang JC, Liao CY, Tien DC, Tsung TT (2009) Preparation of gold ethanol colloid by the arc discharge method. J Alloy Compd 472:446–450. doi:10.1016/j.jallcom.2008.04.084

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants from the China Postdoctoral Science Foundation (No. 20080440811), the National High Technology Research and Development Program of China (863 Program) (No. 2007AA021307), and the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period of China (No. 2007BAD75B05).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China

    Liangwei Du, Liang Xian & Jia-Xun Feng

  2. College of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China

    Liangwei Du

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  1. Liangwei Du
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  2. Liang Xian
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  3. Jia-Xun Feng
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Correspondence to Jia-Xun Feng.

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Du, L., Xian, L. & Feng, JX. Rapid extra-/intracellular biosynthesis of gold nanoparticles by the fungus Penicillium sp.. J Nanopart Res 13, 921–930 (2011). https://doi.org/10.1007/s11051-010-0165-2

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