Summary
The solid material in liquefied mash of cassava tuber was very efficiently separated by a mixture of Trichoderma cellulase and Aspergillus niger pectinase. The solid content of the residue after the treatment and centrifugation decreased from 29.5% to 7.0%. The transparent digested solution from cassava tuber after centrifugation was continuously saccharified by glucoamylase immobilized in a gel which was prepared using polyvinylpyrrolidone and γ-ray irradiation. The addition of 50 ppm of sulfite ion completely prevented microbial contamination during the 18 days of operation. The final DE (dextrose equivalent), glucose content and disaccharide content in the hydrolyzate were 98, 94.4 and 3.3%, respectively.
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References
Inoue T (1981) Report on ethanol production and situation of the raw materials in Brazil and Argentine. Ethanol Production Agency (ed), Ministry of International Trade and Industry, Tokyo, Japan, pp 39–40
Japanese Industrial Standard analytical method k 0102 (1974) Cl−, 80; PO −−−4 , 86, SOCl−, 80; PO −−4 , 101:k 0102 (1981) CN−, 113. Japanese Industrial Standard Committee (ed), Japanese Standard Association, Tokyo, Japan
Kierstan M, Bucke C (1977) The immobilization of microbial cells, subcellular organelles and enzymes in calcium alginate gels. Biotechnol Bioeng 19:387–397
Kobayashi T, Tabuchi T (1954) A method employing a tribasic sodium phosphate buffered reagent for estimating semi-micro quantities of reducing sugars. J Agric Chem Soc Japan 28: 171–174
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265
Maeda H (1975) Preparation of immobilized α-galactosidase by polyvinylpyrrolidone and the continuous hydrolysis of lactose in acid whey. Biotechnol Bioeng 17:1571–1589
Maeda H, Araujo NQ, Reis RF, Silva HGB (1980) Research on liquefaction of cassava. Proceedings of International Symposium on Advanced Industrial Utilization of the Tropical plants. Agency of Industrial Science and Technology (ed), Ministry of International Trade and Industry, Tokyo, Japan, pp 267–273
Maeda H, Chen LF, Tsao GT (1979) Glucoamylase immobilized on diethylaminoethylcellulose beads in column reaction. J Ferment Technol 57:238–243
Maeda H, Suzuki H (1972) Preparation and general properties of glucoamylase bound to halogenacetyl cellulose. Agr Bio Chem 36:1581–1593
Marion L (1979) In the spotlight: ethanol. Chem Eng 86(5):78–80
Menezes TJB, Arakaki T, Delamo PR, Sales AM (1978) Fungal cellulases as an aid for the saccharification of cassava. Biotechnol Bioeng 20:555–565
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Maeda, H., Kajiwara, S. & Araujo, N.Q. The separation of solids from the liquefied mash of cassava tuber and continuous saccharification by immobilized glucoamylase. European J. Appl. Microbiol. Biotechnol. 16, 92–98 (1982). https://doi.org/10.1007/BF00500733
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DOI: https://doi.org/10.1007/BF00500733