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Isolation of microorganisms able to produce 1,3-propanediol and optimization of medium constituents for Klebsiella pneumoniae AJ4

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Abstract

Microbial fermentation under anaerobic and microaerobic conditions has been used for the production of 1,3-propanediol (1,3-PD), a monomer used to produce polymers such as polytrimethylene terephthalate. In this study, we screened microorganisms using the high throughput screening method and isolated the Klebsiella pneumoniae AJ4 strain, which is able to produce 1,3-PD under aerobic conditions. To obtain the maximum 1,3-PD concentration from glycerol, the response surface methodology based on a central composite design was chosen to show the statistical significance of the effects of glycerol, peptone, and (NH4)2SO4 on 1,3-PD production by K. pneumoniae AJ4. The optimal culture medium factors for achieving maximum concentrations of 1,3-PD included glycerol, 108.5 g/L; peptone, 2.72 g/L; and (NH4)2SO4, 4.38 g/L. Under this optimum condition, the maximum concentration of 1,3-PD, 54.76 g/L, was predicted. A concentration of about 52.59 g/L 1,3-PD was obtained using the optimized medium during 26-h batch fermentation, a finding that agreed well with the predicted value.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0025678).

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Authors and Affiliations

  1. Department Molecular Science and Technology, Graduate School of Interdisciplinary Program, Ajou University, Suwon, 443-749, Korea

    Eunsoo Hong, Sangyoung Yoon, Jinyoung Kim, Eumin Kim, Doosub Kim & Yeon-woo Ryu

  2. Department of Food and Nutrition, The University of Suwon, Hwaseong, 445-743, Korea

    Seunggyo Rhie

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  1. Eunsoo Hong
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  2. Sangyoung Yoon
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  3. Jinyoung Kim
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  4. Eumin Kim
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  7. Yeon-woo Ryu
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Correspondence to Yeon-woo Ryu.

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Hong, E., Yoon, S., Kim, J. et al. Isolation of microorganisms able to produce 1,3-propanediol and optimization of medium constituents for Klebsiella pneumoniae AJ4. Bioprocess Biosyst Eng 36, 835–843 (2013). https://doi.org/10.1007/s00449-013-0921-7

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  • DOI: https://doi.org/10.1007/s00449-013-0921-7

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