Abstract
In fed-batch culture of Klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. When the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-products, especially lactic acid and ethanol, decreased sharply. High concentrations of PDO and acetate inhibited cell growth and PDO production. To improve the PDO production, repeated fed-batch culture with addition of the organic acid mixture was performed in a 5-l reactor. The fed-batch culture was repeated five times, and the 1,3-propanediol yield and concentration reached above 0.61 mol mol−1 and 66 g l−1, respectively, in 20 h for each cycle. Furthermore, the PDO productivity reached above 3.30 g l−1 h−1 in each cycle, which was much higher than that of the original fed-batch culture.
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This work was supported by BG2006036, the Department of Science and Technology, Jiangsu Province, China, and partly supported by Shanghai Leading Academic Discipline Project, no. B505.
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Xue, X., Li, W., Li, Z. et al. Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture. J Ind Microbiol Biotechnol 37, 681–687 (2010). https://doi.org/10.1007/s10295-010-0711-z
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DOI: https://doi.org/10.1007/s10295-010-0711-z