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Effects of neuropeptide F on regeneration in Girardia tigrina (Platyhelminthes)

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Abstract

The effects of neuropeptide F (NPF; from Moniezia expansa) on the regeneration of Girardia tigrina were studied. The animals were decapitated and incubated in water (control) or NPF. The dynamics of the proliferation of the neoblasts in the developing tissue were studied during the course of regeneration by monitoring the mitotic index (MI). The effects of incubation in FMRFamide and GYIRFamide on the MI were also tested. The course of cephalic regeneration was followed with in vivo computer-assisted morphometry for up to 7 days. The development of the regenerating nervous system and the musculature was visualised by immunostaining with a primary antiserum to the C-terminal decapeptide of NPF (YFAIIGRPRFa) and tetramethylrhodamine-isothiocyanate-conjugated phalloidin, which stains F-actin in muscle filaments. The study showed that NPF had a stimulatory effect on the mitotic activity of the neoblasts. FMRFamide and GYIRFamide did not have this effect. NPF also stimulated the growth of the regenerating head and the growing nervous system and musculature. NPF is postulated to have a morphogenetic action in the regenerating animals.

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Acknowledgements

We are grateful for the constructive comments of the referees.

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Authors and Affiliations

  1. Institute of Cell Biophysics, Pushchino, Moscow Region, 142290, Russia

    Natalia D. Kreshchenko, Zakhar Sedelnikov & Inna M. Sheiman

  2. Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520, Åbo, Finland

    Maria Reuter & Margaretha K. S. Gustafsson

  3. Parasitology Research Group, School of Biological Sciences, Queen’s University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK

    Aaron G. Maule

Authors
  1. Natalia D. Kreshchenko
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  2. Zakhar Sedelnikov
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  3. Inna M. Sheiman
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  4. Maria Reuter
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  5. Aaron G. Maule
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  6. Margaretha K. S. Gustafsson
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Corresponding author

Correspondence to Margaretha K. S. Gustafsson.

Additional information

This work was supported by two grants from the Finnish Academy of Science (nos. 202685, 2004) and (no. 112090, 2006) to M.G., an RFBR grant (07-04-00452a) to N.K. and a Wellcome Trust grant (069411) to A.G.M. for which we express our gratitude.

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Kreshchenko, N.D., Sedelnikov, Z., Sheiman, I.M. et al. Effects of neuropeptide F on regeneration in Girardia tigrina (Platyhelminthes). Cell Tissue Res 331, 739–750 (2008). https://doi.org/10.1007/s00441-007-0519-y

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