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Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield

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Abstract

Main conclusion

A new wheat-rye 1BL•1RS translocation line, with the characteristics of superior stripe rust resistance and high thousand-kernel weight and grain number per spike, was developed and identified from progenies of wheat-rye- Psathyrostachys huashanica trigeneric hybrids.

Abstract

The wheat-rye 1BL•1RS translocation line from Petkus rye has contributed substantially to the world wheat production. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. In this study, a new wheat-rye line K13-868, derived from the progenies of wheat-rye-Psathyrostachys huashanica trigeneric hybrids, was identified and analyzed using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), acid polyacrylamide gel electrophoresis (A-PAGE), and molecular markers. Cytological studies indicated that the mean chromosome configuration of K13-868 at meiosis was 2n = 42 = 0.14 I + 18.78 II (ring) + 2.15 II (rod). Sequential FISH and GISH results demonstrated that K13-868 was a compensating wheat-rye 1BL•1RS Robertsonian translocation line. Acid PAGE analysis revealed that clear specific bands of rye 1RS were expressed in K13-868. Simple sequence repeat (SSR) and rye 1RS-specific markers ω-sec-p1/ω-sec-p2 and O-SEC5′-A/O-SEC3′-R suggested that the 1BS arm of wheat had been substituted by the 1RS arm of rye. At the seedling and adult growth stage, compared with its recurrent wheat parent SM51 and six other wheat cultivars containing the 1RS arm in southwestern China, K13-868 showed high levels of resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, which are virulent to Yr10 and Yr24/Yr26. In addition, K13-868 expresses higher thousand-kernel weight and more grain number per spike than these controls in two growing seasons, suggesting that this line may carry yield-related genes of rye. This translocation line, with significant characteristics of resistance to stripe rust and high thousand-kernel weight and grain number per spike, could be utilized as a valuable germplasm for wheat improvement.

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Abbreviations

GISH:

Genome in situ hybridization

FISH:

Fluorescence in situ hybridization

SSR:

Simple sequence repeat

Pst :

Puccinia striiformis f. sp. tritici

CS:

Chinese Spring

SM51:

Shumai51

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 31501311), the National High Technology Research and Development Program of China (863 program, No. 2011AA100103), and the Science and Technology Bureau and Education Bureau of Sichuan Province. We thank Dr. Tang ZX (Sichuan Agricultural University, China) for technical guidance in FISH analysis. We also thank Dr. Steven S. Xu, Red River Valley Agricultural Research Center, USDA-ARS, USA, for help with manuscript improvement.

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  1. Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China

    Weiliang Qi, Yao Tang, Wei Zhu, Daiyan Li, Chengdou Diao, Lili Xu, Yi Wang, Xing Fan, Lina Sha, Haiqin Zhang, Youliang Zheng, Yonghong Zhou & Houyang Kang

  2. College of Resources, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China

    Jian Zeng

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  1. Weiliang Qi
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Qi, W., Tang, Y., Zhu, W. et al. Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield. Planta 244, 405–416 (2016). https://doi.org/10.1007/s00425-016-2517-3

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