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Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis

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Abstract

Genomic in situ hybridization (GISH) and multicolor GISH (mcGISH) methodology were used to establish the cytogenetic constitution of five partial amphiploid lines obtained from wheat × Thinopyrum intermedium hybridizations. Line Zhong 1, 2n=52, contained 14 chromosomes from each of the wheat genomes plus ten Th. intermedium chromosomes, with one pair of A-genome chromosomes having a Th. intermedium chromosomal segment translocated to the short arm. Line Zhong 2, 2n=54, had intact ABD wheat genome chromosomes plus 12 Th. intermedium chromosomes. The multicolor GISH results, using different fluorochrome labeled Th. intermedium and the various diploid wheat genomic DNAs as probes, indicated that both Zhong 1 and Zhong 2 contained one pair of Th. intermedium chromosomes with a significant homology to the wheat D genome. High-molecular-weight (HMW) glutenin and gliadin analysis revealed that Zhong 1 and Zhong 2 had identical banding patterns that contained all of the wheat bands and a specific HMW band from Th. intermedium. Zhong 1 and Zhong 2 had good HMW subunits for wheat breeding. Zhong 3 and Zhong 5, both 2n=56, possessed no gross chromosomal aberrations or translocations that were detectable at the GISH level. Zhong 4 also had a chromosome number of 2n=56 and contained the complete wheat ABD-genome chromosomes plus 14 Th. intermedium chromosomes, with one pair of Th. intermedium chromosomes being markedly smaller. Multicolor GISH results indicated that Zhong 4 also contained two pairs of reciprocally translocated chromosomes involving the A and D genomes. Zhong 3, Zhong 4 and Zhong 5 contained a specific gliadin band from Th. intermedium. Based on the above data, it was concluded that inter-genomic transfer of chromosomal segments and/or sequence introgression had occurred in these newly synthesized partial amphiploids despite their diploid-like meiotic behavior and disomic inheritance.

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Acknowledgements

We are grateful to Mr. Zhang Yanbin and Ms. Gao Zhi for technical assistance in the seed storage protein analysis and for maintaining the plants over the years. This study was supported in part by the National Science Fund for Distinguished Young Scholars of China (no. 30225003).

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Author notes

  1. Fangpu Han

    Present address: Division of Biological Sciences, University of Missouri, 117 Tucker Hall, Columbia, MO, 65211-7400, USA

  2. Zhaohui Liu

    Present address: Department of Plant Pathology, North Darkata University, Fargo, ND, 58105, USA

Authors and Affiliations

  1. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, 960 Carling Ave, Bldg 50, Ottawa, ON, Canada, K1A 0C6

    Fangpu Han, George Fedak & Zhaohui Liu

  2. School of Life Sciences, Northeast Normal University, Changchun, 130024, China

    Bao Liu

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  1. Fangpu Han
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  2. Bao Liu
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  3. George Fedak
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  4. Zhaohui Liu
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Correspondence to Fangpu Han or George Fedak.

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Communicated by P. Shewry

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Han, F., Liu, B., Fedak, G. et al. Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor Appl Genet 109, 1070–1076 (2004). https://doi.org/10.1007/s00122-004-1720-y

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