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Expression of a rice glutaredoxin in aleurone layers of developing and mature seeds: subcellular localization and possible functions in antioxidant defense

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Abstract

Main Conclusion

A rice glutaredoxin isoform (OsGrxC2;2) with antioxidant capacity is expressed abundantly in seed tissues and is localized to storage vacuoles in aleurone layers in developing and mature seeds.

Seed tissues undergo drastic water loss at the late stage of seed development, and thus need to tolerate oxidative injuries associated with desiccation. We previously found a rice glutaredoxin isoform, OsGrxC2;2, as a gene expressed abundantly in developing seeds. Since glutaredoxin is involved in antioxidant defense, in the present study we investigated the subcellular localization and expression profile of OsGrxC2;2 and whether OsGrxC2;2 has a role in the defense against reactive oxygen species. Western blotting and immunohistochemistry revealed that the OsGrxC2;2 protein accumulated at a high level in the embryo and aleurone layers of developing and mature seeds. The OsGrxC2;2 in developing seeds was particularly localized to aleurone grains, which are storage organelles derived from vacuoles. Overexpression of OsGrxC2;2 resulted in an enhanced tolerance to menadione in yeast and methyl viologen in green leaves of transgenic rice plants. These results suggest that OsGrxC2;2 participates in the defense against oxidative stress in developing and mature seeds.

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Abbreviations

AsA:

Ascorbate

CaMV:

Cauliflower mosaic virus

DAF:

Days after flowering

DAI:

Days after the onset of imbibition

DHAR:

Dehydroascorbate reductase

ER:

Endoplasmic reticulum

GFP:

Green fluorescent protein

Grx:

Glutaredoxin

GSH:

Glutathione

Prx:

Peroxiredoxin

PS:

Photosystem

RFP:

Red fluorescent protein

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Trx:

Thioredoxin

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (grant no. 18780078 to S.M.), and by a grant from The Tojuro Iijima Foundation for Food Science and Technology (to S.M).

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Author notes

  1. Ayaka Hosoki

    Present address: Radiation Effect Accumulation and Prevention Project, Fukushima Project Headquarters, National Institute of Radiological Sciences, Anagawa, Inage, Chiba, 263-8555, Japan

  2. I. Nengah Suwastika

    Present address: Agricultural Faculty, Tadulako University, Palu, 94118, Indonesia

  3. Shigeru Satoh

    Present address: Faculty of Agriculture, Ryukoku University, Seta, Otsu, 520-2194, Japan

Authors and Affiliations

  1. Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, Kyoto, 606-8522, Japan

    Shigeto Morita, Yuki Yamashita, Masayoshi Fujiki, Rie Todaka, Yuri Nishikawa, Ayaka Hosoki, Chisato Yabe, Jun’ichi Nakamura, Kazuyoshi Kawamura, Takehiro Masumura, Kunisuke Tanaka & Shigeru Satoh

  2. Laboratory of Plant Molecular Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo, Kyoto, 606-8522, Japan

    I. Nengah Suwastika & Masa H. Sato

  3. Biotechnology Research Department, Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center, Seika, Soraku, Kyoto, 619-0244, Japan

    Shigeto Morita, Takehiro Masumura & Shigeru Satoh

  4. Graduate School of Biostudies, Kyoto University, Kyoto, Sakyo, 606-8501, Japan

    I. Nengah Suwastika

  5. Kihara Institute for Biological Research, Yokohama City University, Totsuka, Yokohama, 244-0813, Japan

    Yasunari Ogihara

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Correspondence to Shigeto Morita.

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Morita, S., Yamashita, Y., Fujiki, M. et al. Expression of a rice glutaredoxin in aleurone layers of developing and mature seeds: subcellular localization and possible functions in antioxidant defense. Planta 242, 1195–1206 (2015). https://doi.org/10.1007/s00425-015-2354-9

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