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A peroxiredoxin antioxidant is encoded by a dormancy-related gene,Per1, expressed during late development in the aleurone and embryo of barley grains

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Abstract

Antioxidants can remove damaging reactive oxygen species produced as by-products of desiccation and respiration during late embryogenesis, imbibition of dormant seeds and germination. We have expressed a protein, PER1, encoded by theBalem (barleyaleurone andembryo) transcript previously called B15C, and show it to reduce oxidative damagein vitro. PER1 shares high similarity to a novel group of thiol-requiring antioxidants, named peroxiredoxins, and represents a subgroup with only one conserved cysteine residue (1-Cys). PER1 is the first antioxidant belonging to the 1-Cys subgroup shown to be functionally active, and the first peroxiredoxin of any kind to be functionally described in plants. The steady state level of the transcript,Per1, homologous to a dormancy-related transcript (pBS128) from bromegrass (Bromus secalinus), increases considerably in imbibed embryos from dormant barley (Hordeum vulgare L.) grains. Our investigations also indicate thatPer1 transcript levels are dormancy-related in the aleurone layer of whole grains. In contrast to most seed-expressed antioxidantsPer1 disappears in germinating embryos, and in the mature aleurone the transcript is down-regulated by the germinating embryo or by gibberellic acid (GA). Our data show that the barley seed peroxiredoxin is encoded by a singlePer1 gene. Possible roles of the PER1 peroxiredoxin in barley grains during desiccation, dormancy and imbibition are discussed.

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Authors and Affiliations

  1. Division of General Genetics, University of Oslo, P.O. Box 1031, N-0315, Oslo, Norway

    Robin A. P. Stacy, Else Munthe, Terje Steinum, Babita Sharma & Reidunn B. Aalen

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  1. Robin A. P. Stacy
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Stacy, R.A.P., Munthe, E., Steinum, T. et al. A peroxiredoxin antioxidant is encoded by a dormancy-related gene,Per1, expressed during late development in the aleurone and embryo of barley grains. Plant Mol Biol 31, 1205–1216 (1996). https://doi.org/10.1007/BF00040837

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