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Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

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An Erratum to this article was published on 15 December 2009

Abstract

Stilbene synthase is a plant-specific polyketide synthase, and plays important roles in diverse metabolic processes. The genomic stilbene synthase gene was cloned from accession “Baihe-35-1” of Chinese wild Vitis pseudoreticulata, and a stilbene synthase of V. pseudoreticulata (VpSTS) transcripts expressed in the grape–powdery mildew interaction were determined by semi-quantitative RT-PCR. To monitor VpSTS expression in plant, the promoter region flanking the 5′ VpSTS coding region was isolated from the genomic DNA of Chinese wild V. pseudoreticulata accession Baihe-35-1. Alignment of the VpSTS promoter sequence showed a 56.4% identity to Vitis vinifera. To identify the upstream region of the VpSTS gene required for promoter activity, a series of VpSTS promoter deletion derivatives was constructed. Each deletion construct was analyzed by Agrobacterium-mediated transient transformation in grapevine and tobacco leaves after infection by Uncinula necator and Alternaria alternata. In transiently transformed grapevine leaves, GUS activity was also determined after treatment with salicylic acid (SA) and 4°C cold. Analysis of a series of 5′ deletions of the VpSTS promoter in grapevine leaves indicated that the proximal 162 bp from the transcription initiation site was proved to be necessary for establishing both the constitutive and induced pattern of expression.

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Abbreviations

CaMV35S :

Cauliflower mosaic virus 35S promoter

MES:

2-(N-Morpholino) ethanesulphonic Acid

4-MU:

4-Methylumbelliferone

MUG:

4-Methyl umbelliferyl glucuronide

RT-PCR:

Reverse transcriptase-polymerase chain reaction

VpSTS :

Vitis pseudoreticulata stilbene synthase

GUS:

β-Glucuronidase

SA:

Salicylic acid

X-Gluc:

5-Bromo-4-chloro-3-indolyl-b-d-glucuronic acid

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Acknowledgments

The authors thank Dr. Carole L. Bassett (USDA, ARS, Appalachian Fruit Research Station, Kearneysville, West Virginia, USA) for critical review and comments of the manuscript and Dr. Houhua Li, Dr. Yizhen Wan of Northwest A & F University for helpful advice. This work was supported by the National Natural Science Foundation of China (Grant Nos. 30771493 and 30971972), the National High Technology Research and Development Program of China (863 Program) (Grant No. 2007AA10Z182), and the earmarked fund for Modern Agro-industry Technology Research System.

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Authors and Affiliations

  1. Key Laboratory of Horticulture Plant Germplasm Utilization in Northwest China, Ministry of Agriculture, 712100, Yangling, Shaanxi, China

    Weirong Xu, Yihe Yu, Jiahua Ding, Zhanyong Hua & Yuejin Wang

  2. College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Weirong Xu, Yihe Yu, Jiahua Ding, Zhanyong Hua & Yuejin Wang

  3. Key Laboratory of Agricultural Molecular Biology of Shaanxi Province, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Weirong Xu, Yihe Yu, Jiahua Ding, Zhanyong Hua & Yuejin Wang

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  1. Weirong Xu
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Correspondence to Yuejin Wang.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00425-009-1084-2

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Xu, W., Yu, Y., Ding, J. et al. Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata . Planta 231, 475–487 (2010). https://doi.org/10.1007/s00425-009-1062-8

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