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Silencing of the MADS-Box Gene SlMADS83 Enhances Adventitious Root Formation in Tomato Plants

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Abstract

Adventitious roots (ARs) are important for the growth of plants and the improvement in their stress resistance and survival capacity. Although many genes have been confirmed to be involved in adventitious root (AR) formation in Arabidopsis and tomato plants, MADS-box genes have rarely been mentioned. Here, we isolated a MADS-box gene named SlMADS83, which may negatively regulate AR formation in tomato plants, as the number of the ARs formed in the transgenic lines in which the SlMADS83 gene was silenced by RNA interference (RNAi) was increased. The above phenotype was further confirmed by the analysis of the macroscopic, anatomical, and molecular features and related statistical data. Previous Studies have proven that auxin can stimulate early AR primordium initiation. Interestingly, in the RNAi transgenic lines, the concentration of auxin in the hypocotyl base was increased, resulting in early induction of AR primordia initiation, promoting the formation of ARs. Briefly, SlMADS83 may play an important role in AR formation.

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Abbreviations

RNAi:

RNA interference

IAA:

Indole-3-acetic acid

ACC:

1-Aminocyclopropane-1-carboxylate

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Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 31572129) and the Natural Science Foundation of Chongqing of China (No. cstc2015jcyjA80026).

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Authors and Affiliations

  1. Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing, 400044, People’s Republic of China

    Anzhou Li, Guoping Chen, Yunshu Wang, Honglian Liang & Zongli Hu

  2. Bioengineering College, Chongqing University, Room 523-1, Campus B, 174 Shapingba Main Street, Chongqing, 400030, People’s Republic of China

    Zongli Hu

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  1. Anzhou Li
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Z.H. and G.C. designed the research; A.L., Y.W., and H.L. performed the research; A.L. wrote the paper; Z.H. modified the paper. All authors have read and approved the final version of the manuscript.

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Correspondence to Zongli Hu.

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Supplementary material 2 (DOCX 433 kb) Supplementary Fig. S1. Phylogenetic analyses and gene expression prediction.

Supplementary material 3 (DOCX 19 kb) Supplementary Table S1. Specific primer sequences used in this study.

344_2019_10035_MOESM4_ESM.docx

Supplementary material 4 (DOCX 16 kb) Supplementary Table S2. Relative potential MADS binding sites (GArG motifs) in the promoter sequences.

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Li, A., Chen, G., Wang, Y. et al. Silencing of the MADS-Box Gene SlMADS83 Enhances Adventitious Root Formation in Tomato Plants. J Plant Growth Regul 39, 941–953 (2020). https://doi.org/10.1007/s00344-019-10035-w

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