Abstract
ASYMMETRIC LEAVES2-LIKE16 gene of Arabidopsis is a member of the ASYMMETRIC LEAVES2 (AS2)/LATERAL ORGAN BOUNDARIES (LOB) domain gene family. Current reports have indicated that overexpression of ASL16 induces lateral root formation in the absence of ARF7 and ARF19; whereas lateral root formation is severely reduced in the arf 7 and arf 19 double knockout mutants. Antisense mRNA has testified to be a useful tool to inhibit the expression of specific genes in plants; more significantly, it is specific in the sense that only the mRNA level of the desired protein being changed, while other members of the gene family remain unaffected. To further explore ASL16 function in lateral root growth and development, we introduced an antisense-expression construct of the ASL16 gene to Arabidopsis (Col-0); and acquired some 35S:ASL16 Arabidopsis plants. With 11 day after germination (DAG), many lateral roots in wild-type seedlings were well developed, whereas a few lateral roots in these antisense 35S:ASL16 seedlings were produced. However, with 5, 8 and 16 DAG, the lateral roots in these transgenic seedlings were not distinct with those of wild-type plants. A series of data indicate that primary root formation of these antisense 35S:ASL16 plants was not affected; whereas their lateral root formation was severely inhibited.
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Abbreviations
- RT-PCR:
-
reverse transcription PCR
- WT:
-
wild type
- M:
-
marker
- DAG:
-
day after germination
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Meng, LS., Bizhu, H., Cao, XY. et al. ASL16 gene, a member of AS2/LOB family, is essential for lateral root formation in Arabidopsis. Biologia 71, 1009–1016 (2016). https://doi.org/10.1515/biolog-2016-0120
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DOI: https://doi.org/10.1515/biolog-2016-0120