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ZmHSP16.9, a cytosolic class I small heat shock protein in maize (Zea mays), confers heat tolerance in transgenic tobacco

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Abstract

Various organisms produce HSPs in response to high temperature and other stresses. The function of heat shock proteins, including small heat shock protein (sHSP), in stress tolerance is not fully explored. To improve our understanding of sHSPs, we isolated ZmHSP16.9 from maize. Sequence alignments and phylogenetic analysis reveal this to be a cytosolic class I sHSP. ZmHSP16.9 expressed in root, leaf and stem tissues under 40 °C treatment, and was up-regulated by heat stress and exogenous H2O2. Overexpression of ZmHSP16.9 in transgenic tobacco conferred tolerance to heat and oxidative stresses by increased seed germination rate, root length, and antioxidant enzyme activities compared with WT plants. These results support the positive role of ZmHSP16.9 in response to heat stress in plant.

Key message The overexpression of ZmHSP16.9 enhanced tolerance to heat and oxidative stress in transgenic tobacco.

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Acknowledgments

This work was supported by the State Key Basic Research and Development Plan of China (2009CB118500), the National Natural Sciences Foundation of China (30970229), the Research Fund for the Doctoral Program of Higher Education of China (20103702110007) and the foundation of State Key Laboratory of Crop Biology (2010KF11).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 Daizong Street, Tai’an, 271018, Shandong, China

    Liping Sun, Yang Liu, Xiangpei Kong, Dan Zhang, Jiaowen Pan, Yan Zhou, Li Wang, Dequan Li & Xinghong Yang

  2. Taishan Medical University, Tai’an, 271000, Shandong, China

    Liping Sun

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  1. Liping Sun
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  2. Yang Liu
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  3. Xiangpei Kong
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  4. Dan Zhang
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  6. Yan Zhou
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  7. Li Wang
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  9. Xinghong Yang
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Correspondence to Xinghong Yang.

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Communicated by K. Chong.

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Sun, L., Liu, Y., Kong, X. et al. ZmHSP16.9, a cytosolic class I small heat shock protein in maize (Zea mays), confers heat tolerance in transgenic tobacco. Plant Cell Rep 31, 1473–1484 (2012). https://doi.org/10.1007/s00299-012-1262-8

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  • DOI: https://doi.org/10.1007/s00299-012-1262-8

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