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Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.)

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Abstract

The cytosolic class I small heat shock proteins (sHSP-CI) represent the most abundant sHSP in plants. Here, we report the characterization and the expression profile of nine members of the sHSP-CI gene family in rice (Oryza sativa Tainung No.67), of which Oshsp16.9A, Oshsp16.9B, Oshsp16.9C, Oshsp16.9D and Oshsp17.9B are clustered on chromosome 1, and Oshsp17.3, Oshsp17.7, Oshsp17.9A and Oshsp18.0 are clustered on chromosome 3. Oshsp17.3 and Oshsp18.0 are linked by a 356-bp putative bi-directional promoter. Individual gene products were identified from the protein subunits of a heat shock complex (HSC) and from in vitro transcription/ translation products by two-dimensional gel electrophoreses (2-DE). All sHSP-CI genes except Oshsp17.9B were induced strongly after a 2-h heat shock treatment. The genes on chromosome 3 were induced rapidly at 32  and 41 °C, whereas those on chromosome 1 were induced slowly by similar conditions. Seven of these genes, except Oshsp16.9D and Oshsp17.9B, were induced by arsenite (As), but only genes on chromosome 3 were strongly induced by azetidine-2-carboxylic acid (Aze, a proline analog) and cadmium (Cd). A similar expression profile of all sHSP-CI genes at a lower level was evoked by ethanol, H2O2 and CuCl2 treatments. Transient expression assays of the promoter activity by linking to GUS reporter gene also supported the in vivo selective expression of the sHSP-CI genes by Aze treatment indicating the differential induction of rice sHSP-CI genes is most likely regulated at the transcriptional level. Only Oshsp16.9A abundantly accumulated in mature dry seed also suggested additionally prominent roles played by this HSP in development.

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Abbreviations

ACD:

α-crystallin domain

As:

arsenite

Aze:

L-azetidine-2-carboxlyic acid

Cd:

cadmium

GUS:

β-glucuronidase

HSC:

heat shock complex

HSE:

heat shock response element

ROS:

reactive oxygen species

sHSP:

small heat shock protein

sHSP-CI:

class I small heat shock protein

2-DE:

two-dimensional gel electrophoresis

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Authors and Affiliations

  1. Institute of Plant Biology, National Taiwan University, Taipei, 106, Taiwan, ROC

    Jiahn-Chou Guan, Tsung-Luo Jinn, Shi-Pin Feng, Yih-Ming Chen & Chu-Yung Lin

  2. Department of Life Science, National Central University, Taiwan, ROC

    Ching-Hui Yeh

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  1. Jiahn-Chou Guan
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  2. Tsung-Luo Jinn
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  3. Ching-Hui Yeh
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  4. Shi-Pin Feng
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  5. Yih-Ming Chen
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  6. Chu-Yung Lin
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Correspondence to Chu-Yung Lin.

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Guan, JC., Jinn, TL., Yeh, CH. et al. Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.). Plant Mol Biol 56, 795–809 (2004). https://doi.org/10.1007/s11103-004-5182-z

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