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Isolation and Characterization of a Furfural-Degrading Bacterium Bacillus cereus sp. Strain DS1

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Abstract

Furfural was found to be the main organic pollutant in the wastewater coming from the Diosgenin factory. This substance is derived from acidic pentosan in Dioscorea zingiberensis and is also found in a variety of agricultural byproducts, including corncobs, oat, wheat bran, and sawdust. It is regarded as a toxicant and an inhibitor to the growth of microorganism in both sewage disposal and biological fermentation. A furfural-degrading strain (DS1) was isolated from activated sludge of wastewater treatment plant in a diosgenin factory by continuous enrichment culture. The strain was identified as Bacillus cereus based on morphological, physiological tests, as well as on 16S rDNA sequence and Biolog analyses. The capacity of this strain to grow on a mineral salt medium, utilizing furfural as the sole carbon and energy source to degrade furfural, was investigated in this study. Under the condition of pH 9.0, temperature 35 °C, with rotating speed of 150 rpm, and an inoculum of 6 %, the strain showed that the furfural degradation capacity reaches 35 % in 7 days, as measured by high-performance liquid chromatography. The addition of inorganic carbon sources could bring down the biodegradation efficiency of the furfural. The strain DS1 showed better furfural removal capacity, as compared to other inorganic carbon sources in the media. Furthermore, a furfural concentration of as high as 4,000 mg L−1 was tolerated by the culture. The capacity to degrade furfural was demonstrated for the first time by using the genus B. cereus. This study suggests the possible application in biodegradation strategies.

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Acknowledgments

This research was supported by the National Key Technologies R&D Program (No. 2006BAB04A14) and the Hubei Provincial Science and Technology Department (No. 2006AA305A05).

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Authors and Affiliations

  1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Dan Zheng, Jianguo Bao, Jueming Lu & Chunlei Gao

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  1. Dan Zheng
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  2. Jianguo Bao
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  3. Jueming Lu
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  4. Chunlei Gao
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Correspondence to Jianguo Bao.

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Zheng, D., Bao, J., Lu, J. et al. Isolation and Characterization of a Furfural-Degrading Bacterium Bacillus cereus sp. Strain DS1. Curr Microbiol 70, 199–205 (2015). https://doi.org/10.1007/s00284-014-0702-9

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  • DOI: https://doi.org/10.1007/s00284-014-0702-9

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