Abstract
Strains of the yeast genus Blastobotrys (subphylum Saccharomycotina) represent a valuable biotechnological resource for basic biochemistry research, single-cell protein, and heterologous protein production processes. Species of this genus are dimorphic, non-pathogenic, thermotolerant, and can assimilate a variety of hydrophilic and hydrophobic substrates. These can constitute a single-cell oil platform in an emerging bio-based economy as oleaginous traits have been discovered recently. However, the regulatory network of lipogenesis in these yeasts is poorly understood. To keep pace with the growing market demands for lipid-derived products, it is critical to understand the lipid biosynthesis in these unconventional yeasts to pinpoint what governs the preferential channelling of carbon flux into lipids instead of the competing pathways. This review summarizes information relevant to the regulation of lipid metabolic pathways and prospects of metabolic engineering in Blastobotrys yeasts for their application in food, feed, and beyond, particularly for fatty acid-based fuels and oleochemicals.
Key points
• The production of biolipids by heterotrophic yeasts is reviewed.
• Summary of information concerning lipid metabolism regulation is highlighted.
• Special focus on the importance of diacylglycerol acyltransferases encoding genes in improving lipid production is made.
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Authors acknowledge Hiroko KITAMOTO, Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organisation, Tsukuba, Ibaraki, Japan, and Zongbao Kent Zhao, Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023 People’s Republic of China and Mahesh Khot from FONDECYT (Fondo Nacional de Desarrollo Científico y Tecnológico/National Fund for Scientific and Technological Development), Government of Chile, Chile, Santiago in Postdoctoral Project Grant context (No. 3180134), for proofreading this review document.
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Sanya, D.R.A., Onésime, D., Passoth, V. et al. Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production. Appl Microbiol Biotechnol 105, 4879–4897 (2021). https://doi.org/10.1007/s00253-021-11354-3
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DOI: https://doi.org/10.1007/s00253-021-11354-3