Abstract
A new yeast species, Trichosporon adeninovorans, was isolated from soil by the enrichment culture method. Apart from adenine, the strain utilized uric acid, guanine, xanthine, hypoxanthine, 6,8-dihydroxypurine, putrescine, propylamine, butylamine, pentylamine, hexylamine and octylamine as sole source of carbon, nitrogen and energy.
The structure of the cell wall of Tr. adeninovorans was ascomycetous. On the subcellular level growth on adenine or uric acid was accompanied with the development of microbodies in the cell. These cell organelles probably were the site of urate oxidase, an enzyme that, after growth on purine substrates, together with allantoinase was present at high activities. Low activities of adenine amidohydrolase and xanthine dehydrogenase were also demonstrated.
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References
Bergmann, F., Levene, L. and Govrin, H. 1977. Oxidation of hypoxanthines, bearing 8-aryl or 8-pyridyl substituents, by bovine milk xanthine oxidase. — Biochim. Biophys. Acta 484: 275–289.
Bos, P. and De Bruyn J. C. 1973. The significance of hydrocarbon assimilation in yeast identification. — Antonie van Leeuwenhoek 39: 99–107.
DeMoss, R. D. and Bard, R. C. 1957. Physiological and biochemical technics. p. 169–198. In Society of American Bacteriologists (ed.), Manual of Microbiological Methods. — McGraw-Hill Book Company, New York.
Diddens, H. A. and Lodder, J. 1942. Die anaskosporogenen Hefen. Zweite Hälfte. — Noord-Hollandsche Uitgevers Maatschappij, Amsterdam.
Do Carmo-Sousa, L. 1970. Genus Trichosporon Behrend. p. 1309–1352. In J. Lodder (ed.), The Yeasts—A Taxonomic Study, Second edition. — North-Holland Publ. Co., Amsterdam.
Kreger-Van Rij, N. J. W. and Veenhuis, M. 1971. A comparative study of the cell wall structure of basidiomycetous and related yeasts. — J. Gen. Microbiol. 68: 87–95.
Middelhoven, W. J. 1977. Isolation and characterization of methylammonium-resistant mutants of Saccharomyces cerevisiae with relieved nitrogen metabolite repression of allantoinase, arginase and ornithine transaminase synthesis. — J. Gen. Microbiol. 100: 257–269.
Middelhoven, W. J., Van den Brink, J. A. and Veenhuis, M. 1983. Growth of Candida famata and Trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts. — Antonie van Leeuwenhoek 49: 361–368.
Robins, R. K. 1958. Potential purine antagonists. XV. Preparation of some 6,8-disubstituted purines. — J. Am. Chem. Soc. 80: 6671–6679.
Van der Walt, J. P., 1970. Criteria and methods used in classification. p. 34–113. In J. Lodder (ed.), The Yeasts—A Taxonomic Study, Second edition. — North-Holland Publ. Co., Amsterdam.
Van der Walt, J. P. and Johannsen, E. 1975. Trichosporon terrestre sp.nov. — Antonie van Leeuwenhoek 41: 361–365.
Veenhuis, M., Hoogkamer-Te Niet, M. C. and Middelhoven, W. J. 1985. Biogenesis and metabolic significance of microbodies in urate-utilizing yeasts. —Antonie van Leeuwenhoek 51 (in press).
Vogels, G. D. and Van der Drift, C. 1976. Degradation of purines and pyrimidines by microorganisms. — Bacteriol. Rev. 40: 403–468.
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Middelhoven, W.J., Niet, M.C.HT. & Rij, N.J.W.KV. Trichosporon adeninovorans sp. nov., a yeast species utilizing adenine, xanthine, uric acid, putrescine and primary n-alkylamines as the sole source of carbon, nitrogen and energy. Antonie van Leeuwenhoek 50, 369–378 (1984). https://doi.org/10.1007/BF00394651
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DOI: https://doi.org/10.1007/BF00394651