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Principles, techniques, and applications of biocatalyst immobilization for industrial application

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Abstract

Immobilization is one of the most effective and powerful tools used in industry, which has been studied and improved since the last century. Various immobilization techniques and support materials have been used on both laboratory and industrial scale. Each immobilization technique is applicable for a specific production mostly depending on the cost and sensibility of process. Compared to free biocatalyst systems, immobilization techniques often offer better stability, increased activity and selectivity, higher resistance, improved separation and purification, reuse of enzymes, and consequently more efficient process. Recently, many reviews have been published about immobilization systems; however, most of them have focused on a specific application or not emphasized in details. This review focuses on most commonly used techniques in industry with many recent applications including using bioreactor systems for industrial production. It is also aimed to emphasize the advantages and disadvantages of the immobilization techniques and how these systems improve process productivity compared to non-immobilized systems.

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Acknowledgments

Authors thank the Coordenação de Aperfeiçoamento de Pessoas de Nível Superior (CAPES) for the scholarship for Ismail Eş.

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Authors and Affiliations

  1. Laboratory of Nanotechnology and Biotechnology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, S/N, Setor Universitario, Goiânia, GO, Brazil

    Ismail Eş & André Corrêa Amaral

  2. Molecular Biology and Genetics Post Graduation Program, Institute of Biological Science, Federal University of Goiás, Goiânia, GO, Brazil

    Ismail Eş & André Corrêa Amaral

  3. Department of Microbiology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil

    José Daniel Gonçalves Vieira

Authors
  1. Ismail Eş
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  2. José Daniel Gonçalves Vieira
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  3. André Corrêa Amaral
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Correspondence to André Corrêa Amaral.

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Eş, I., Vieira, J.D.G. & Amaral, A.C. Principles, techniques, and applications of biocatalyst immobilization for industrial application. Appl Microbiol Biotechnol 99, 2065–2082 (2015). https://doi.org/10.1007/s00253-015-6390-y

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  • DOI: https://doi.org/10.1007/s00253-015-6390-y

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