Skip to main content
SpringerLink
Log in

Biodiesel production in a magnetically-stabilized, fluidized bed reactor with an immobilized lipase in magnetic chitosan microspheres

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Biodiesel production by immobilized Rhizopus oryzae lipase in magnetic chitosan microspheres (MCMs) was carried out using soybean oil and methanol in a magnetically-stabilized, fluidized bed reactor (MSFBR). The maximum content of methyl ester in the reaction mixture reached 91.3 (w/v) at a fluid flow rate of 25 ml/min and a magnetic field intensity of 150 Oe. In addition, the MCMs-immobilized lipase in the reactor showed excellent reusability, retaining 82 % productivity even after six batches, which was much better than that in a conventional fluidized bed reactor. These results suggested that a MSFRB using MCMs-immobilized lipase is a promising method for biodiesel production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bahar T, Çelebi SS (2000) Performance of immobilized glucoamylase in magnetically stabilized bed reactor (MSFBR). Enz Microb Technol 26:28–33

    Article  CAS  Google Scholar 

  • Bornscheuer UT (2003) Immobilizing enzymes: how to create more suitable biocatalysts. Angew Chem Int Ed 42:3336–3337

    Article  CAS  Google Scholar 

  • Chana HT, Doa YY, Huang PL (2006) Preparation and properties of biocompatible magnetic Fe3O4 nanoparticles. J Magn Magn Mater 304:415–417

    Article  Google Scholar 

  • Chen GY, Ying M, Li WZ (2006) Enzymatic conversion of waste cooking oils into alternative fuel-biodiesel. Appl Biochem Biotechnol 129–132:911–921

    Article  PubMed  Google Scholar 

  • Du W, Wang L, Liu DH (2007) Improved methanol tolerance during Novozym435-mediated methanolysis of SODD for biodiesel production. Green Chem 9:173–176

    Article  CAS  Google Scholar 

  • Fukuda H, Kondo A, Noda H (2001) Biodiesel fuel production by transesterification of oils. Biosci Bioeng 92:405–416

    CAS  Google Scholar 

  • Guo PM, Huang FH, Huang QD, Zheng C (2012) Optimization of biodiesel production using a magnetically stabilized fluidized bed reactor. J Am Oil Chem Soc 89:497–504

    Article  CAS  Google Scholar 

  • Hu B, Pan J, Yu HL, Liu JW, Xu JH (2009) Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic. Proc Biochem 44:1019–1024

    Article  CAS  Google Scholar 

  • Iso M, Chen BX, Eguchi M et al (2001) Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase. Mol Catal B 16:53–58

    Article  CAS  Google Scholar 

  • Jiang DS, Long SY, Huang J, Xiao HY, Zhou JY (2005) Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres. Bioch Eng 25:15–23

    Article  Google Scholar 

  • Orrego CE, Valencia JS, Zapata C (2009) Candida rugosa lipase supported on high crystallinity chitosan as biocatalyst for the synthesis of 1-butyl oleate. Catal Lett 129:312–322

    Article  CAS  Google Scholar 

  • Tong XD, Sun Y (2003) Application of magnetic agarose support in liquid magnetically stabilized fluidized bed for protein adsorption. Biotechnol Prog 19:1721–1727

    Article  CAS  PubMed  Google Scholar 

  • Wardle DA (2003) Global sale of green air travel supported using biodiesel. Renew Sustain Energy Rev 7:1–64

    Article  Google Scholar 

Download references

Acknowledgments

This paper is financially supported by the National High-tech R&D Program (863 program) of China through project (2012AA051801) and the National Natural Science Foundation of China through project (51036006).

Author information

Authors and Affiliations

  1. State Key Laboratory of Engines, School of Environmental Science and Technology, Tianjin University, Tianjin, 300072, China

    Gui-xiong Zhou, Guan-yi Chen & Bei-bei Yan

Authors
  1. Gui-xiong Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guan-yi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bei-bei Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guan-yi Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhou, Gx., Chen, Gy. & Yan, Bb. Biodiesel production in a magnetically-stabilized, fluidized bed reactor with an immobilized lipase in magnetic chitosan microspheres. Biotechnol Lett 36, 63–68 (2014). https://doi.org/10.1007/s10529-013-1336-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-013-1336-x

Keywords

Search

Navigation