Abstract
α-Amylase was immobilized on Dowex MAC-3 with 88 % yield and amyloglucosidase on Amberlite IRA-400 ion-exchange resin beads with 54 % yield by adsorption process. Immobilized enzymes were characterized to measure the kinetic parameters and optimal operational parameters. Optimum substrate concentration and temperature were higher for immobilized enzymes. The thermal stability of the enzymes enhanced after the immobilization. Immobilized enzymes were used in the hydrolysis of the natural starch at high concentration (35 % w/v). The time required for liquefaction of starch to 10 dextrose equivalent (DE) and saccharification of liquefied starch to 96 DE increased. Immobilized enzymes showed the potential for use in starch hydrolysis as done in industry.
Similar content being viewed by others
References
Souza PMD, Magalhães PDOE (2010) Braz J Microbiol 41:850–861
Vihinen M, Mäntsälä P (1990) Biotechnol Appl Biochem 12:427–435
Richetti A, Munaretto CB, Lerin LA, Batistella L, Oliveira VJ, Dallago RM, Astolfi V, Luccio MD, Mazutti MA, Oliveira DD, Treichel H (2012) Bioprocess Biosyst Eng 35:383–388
Bayramoglu G, Yilmaz M, Yakup Arica M (2010) Bioprocess Biosyst Eng 35:439–448
Švec F, Kálal J, Menyailova II, Nakhapetyan LA (1978) Biotechnol Bioeng 20:1319–1328
Lai J-T, Wu S-C, Liu H-S (1998) Bioprocess Eng 18:155–161
Oh JT, Kim JH (2000) Enzyme Microb Technol 27:356–361
Ashly PC, Mohanan PV (2010) Process Biochem 45:1422–1426
Silva RN, Asquieri ER, Fernandes KF (2005) Process Biochem 40:1155–1159
Khan M, Husain Q, Azam A (2012) Biotechnol Bioprocess Eng 17:377–384
Wang J, Zhao G, Li Y, Liu X, Hou P (2013) Appl Microbiol Biotechnol 97:681–692
Shah C, Sellappan S, Madamwar D (2000) Process Biochem 35:971–975
Kikani BA, Pandey S, Singh SP (2012) Bioprocess Biosyst Eng 1–11 (Epub ahead of print)
Jaiswal N, Prakash O, Talat M, Hasan SH, Pandey RK (2012) J Genet Eng Biotechnol 10:161–167
Tanriseven A, Ölçer Z (2008) Biochem Eng J 39:430–434
Miller GL (1959) Anal Chem 31:426–428
Lowry BH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275
Crabb WD, Shetty JK (1999) Curr Opin Microbiol 2:252–256
Lane JH, Eynon L (1923) J Soc Chem Ind 42:32T–37T
Abdel-Naby MA, Hashem AM, Esawy MA, Abdel-Fattah AF (1999) Microbiol Res 153:319–325
Ahmed SA, Mahdy ESME, Hassan O-K (2008) Aust J Basic Appl Sci 2:466–474
Kumari A, Kayastha AM (2011) J Mol Catal B Enzyme 69:8–14
Yagar H, Ertan F, Balkan B (2007) Prep Biochem Biotechnol 38:13–23
Pandya PH, Jasra RV, Newalkar BL, Bhatt PN (2005) Microporous Mesoporous Mater 77:67–77
El-Batal AI, Atia KS, Eid M (2005) Radiat Phys Chem 74:96–101
Kara A, Osman B, Yavuz H, Beşirli N, Denizli A (2005) React Funct Polym 62:61–68
Liu H, Liu J, Tan B, Zhou F, Qin Y, Yang R (2012) Bioprocess Biosyst Eng 35:1287–1295
Dhingra S, Khanna M, Pundir CS (2006) Indian Chem Tech 13:119–121
Sardar M, Agarwal R, Kumar A, Gupta MN (1997) Enzyme Microb Technol 20:361–367
Bayramoglu G, Altintas B, Arica MY (2013) Appl Microbiol Biotechnol 97:1149–1159
Tardioli PW, Vieira MF, Vieira AMS, Zanin GM, Betancor L, Mateo C, Fernández-Lorente G, Guisán JM (2011) Process Biochem 46:409–412
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gupta, K., Jana, A.K., Kumar, S. et al. Immobilization of α-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration. Bioprocess Biosyst Eng 36, 1715–1724 (2013). https://doi.org/10.1007/s00449-013-0946-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-013-0946-y