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Metabolic reconstruction and flux analysis of industrial Pichia yeasts

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Abstract

Pichia yeasts have been recognized as important microbial cell factories in the biotechnological industry. Notably, the Pichia pastoris and Pichia stipitis species have attracted much research interest due to their unique cellular physiology and metabolic capability: P. pastoris has the ability to utilize methanol for cell growth and recombinant protein production, while P. stipitis is capable of assimilating xylose to produce ethanol under oxygen-limited conditions. To harness these characteristics for biotechnological applications, it is highly required to characterize their metabolic behavior. Recently, following the genome sequencing of these two Pichia species, genome-scale metabolic networks have been reconstructed to model the yeasts’ metabolism from a systems perspective. To date, there are three genome-scale models available for each of P. pastoris and P. stipitis. In this mini-review, we provide an overview of the models, discuss certain limitations of previous studies, and propose potential future works that can be conducted to better understand and engineer Pichia yeasts for industrial applications.

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Acknowledgments

This work was supported by the National University of Singapore, the Biomedical Research Council of A*STAR (Agency for Science, Technology and Research), the National Research Foundation of Singapore (NRFCRP5-2009-03), and a grant from the Global R&D project program (N011500017), Ministry of Knowledge Economy, Republic of Korea.

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  1. Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Centros, Singapore, 138668, Singapore

    Bevan Kai-Sheng Chung, Maximilian Klement & Dong-Yup Lee

  2. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117576, Singapore

    Meiyappan Lakshmanan, Maximilian Klement, Chi Bun Ching & Dong-Yup Lee

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  1. Bevan Kai-Sheng Chung
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Chung, B.KS., Lakshmanan, M., Klement, M. et al. Metabolic reconstruction and flux analysis of industrial Pichia yeasts. Appl Microbiol Biotechnol 97, 1865–1873 (2013). https://doi.org/10.1007/s00253-013-4702-7

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