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Flux Balance Analysis: Interrogating Genome-Scale Metabolic Networks

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Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 500))

Summary

Flux balance analysis (FBA) is a computational method to analyze reconstructions of biochemical networks. FBA requires the formulation of a biochemical network in a precise mathematical framework called a stoichiometric matrix. An objective function is defined (e.g., growth rate) toward which the system is assumed to be optimized. In this chapter, we present the methodology, theory, and common pitfalls of the application of FBA.

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Acknowledgments

The authors wish to thank the National Science Foundation CAREER program (grant# 0643548 to JP) for financial support. MAO and AKC contributed equally to this work.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Virginia, Box 800759, Health System, Charlottesville, VA, 22908

    Jason A. Papin

Authors
  1. Matthew A. Oberhardt
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  2. Arvind K. Chavali
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  3. Jason A. Papin
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Correspondence to Jason A. Papin .

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    © 2009 Humana Press

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    Oberhardt, M., Chavali, A., Papin, J. (2009). Flux Balance Analysis: Interrogating Genome-Scale Metabolic Networks. In: Maly, I. (eds) Systems Biology. Methods in Molecular Biology, vol 500. Humana Press. https://doi.org/10.1007/978-1-59745-525-1_3

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    • DOI: https://doi.org/10.1007/978-1-59745-525-1_3

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-64-0

    • Online ISBN: 978-1-59745-525-1

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