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Molecular tools for deciphering the microbial community structure and diversity in rumen ecosystem

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Abstract

Rumen microbial community comprising of bacteria, archaea, fungi, and protozoa is characterized not only by the high population density but also by the remarkable diversity and the most complex microecological interactions existing in the biological world. This unprecedented biodiversity is quite far from full elucidation as only about 15–20 % of the rumen microbes are identified and characterized till date using conventional culturing and microscopy. However, the last two decades have witnessed a paradigm shift from cumbersome and time-consuming classical methods to nucleic acid-based molecular approaches for deciphering the rumen microbial community. These techniques are rapid, reproducible and allow both the qualitative and quantitative assessment of microbial diversity. This review describes the different molecular methods and their applications in elucidating the rumen microbial community.

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Acknowledgments

Authors gratefully acknowledge the National Agricultural Innovation Project, Indian Council of Agriculture Research (ICAR), Government of India for providing financial support during this study. “We thank Dr Joan Edwards for providing useful research materials

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Authors and Affiliations

  1. Nutrition Biotechnology Laboratory, Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Sunil Kumar Sirohi, Nasib Singh, Sumit Singh Dagar & Anil Kumar Puniya

  2. Division of Dairy Microbiology, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Anil Kumar Puniya

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  1. Sunil Kumar Sirohi
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Correspondence to Sunil Kumar Sirohi.

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Sirohi, S.K., Singh, N., Dagar, S.S. et al. Molecular tools for deciphering the microbial community structure and diversity in rumen ecosystem. Appl Microbiol Biotechnol 95, 1135–1154 (2012). https://doi.org/10.1007/s00253-012-4262-2

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