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Properties and applications of microbial β-D-xylosidases featuring the catalytically efficient enzyme from Selenomonas ruminantium

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Abstract

Xylan 1,4-β-D-xylosidase catalyzes hydrolysis of non-reducing end xylose residues from xylooligosaccharides. The enzyme is currently used in combination with β-xylanases in several large-scale processes for improving baking properties of bread dough, improving digestibility of animal feed, production of d-xylose for xylitol manufacture, and deinking of recycled paper. On a grander scale, the enzyme could find employment alongside cellulases and other hemicellulases in hydrolyzing lignocellulosic biomass so that reaction product monosaccharides can be fermented to biofuels such as ethanol and butanol. Catalytically efficient enzyme, performing under saccharification reactor conditions, is critical to the feasibility of enzymatic saccharification processes. This is particularly important for β-xylosidase which would catalyze breakage of more glycosidic bonds of hemicellulose than any other hemicellulase. In this paper, we review applications and properties of the enzyme with emphasis on the catalytically efficient β-d-xylosidase from Selenomonas ruminantium and its potential use in saccharification of lignocellulosic biomass for producing biofuels.

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Acknowledgments

This work was supported by United States Department of Agriculture CRIS 5325-41000-046-00 (K.W.) and CRIS 3620-41000-118-00D (D.B.J.). The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned.

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  1. USDA Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, 61604, USA

    Douglas B. Jordan

  2. USDA Agricultural Research Service, Western Regional Research Center, Albany, CA, 94710, USA

    Kurt Wagschal

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Jordan, D.B., Wagschal, K. Properties and applications of microbial β-D-xylosidases featuring the catalytically efficient enzyme from Selenomonas ruminantium . Appl Microbiol Biotechnol 86, 1647–1658 (2010). https://doi.org/10.1007/s00253-010-2538-y

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