Abstract
Three recombinant inbred line populations from the crosses RL6071/Thatcher, RL6071/RL6058 (Thatcher Lr34), and Thatcher/RL6058, were used to study the genetics of stem rust resistance in Thatcher and TcLr34. Segregation of stem rust response in each population was used to determine the number of genes conferring resistance, as well as the effect of the leaf rust resistance gene Lr34 on stem rust resistance. The relationship between resistance in seedling and adult plants was also examined, and an attempt was made to identify microsatellite markers linked to genes that were effective in adult plants. In field plot tests at least three additive resistance genes segregated in the RL6071/RL6058 population, whereas two resistance genes segregated in the RL6071/Thatcher population. The presence of the gene Lr34 permitted the expression of additional stem rust resistance in Thatcher-derived lines both at the seedling and adult plant stages. Seedling resistance to races TPMK and RKQQ was significantly associated with resistance in adult plants, whereas seedling resistance to races QCCD and QCCB may have made a minor contribution. The seedling resistance genes Sr16 and Sr12 may have contributed to resistance in adult plants. A molecular marker linked to resistance in adult plants was identified on chromosome 2BL.
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Abbreviations
- IT:
-
Infection type
References
Bassam BJ, Caetano-Anollés G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83
Brennan PS (1975) General resistance in wheat (Triticum aestivum L.) to wheat stem rust (Puccinia graminis Pers. f. sp. tritici Erick. and Henn.). Dissertation, University of Saskatchewan, Saskatoon, Canada
Dyck PL (1987) The association of a gene for leaf rust resistance with the chromosome 7D supressor of stem rust resistance in common wheat. Genome 29:467–469
Dyck PL (1993) Inheritance of leaf rust and stem rust resistance in ‘Roblin’ wheat. Genome 36:289–291
Hayes HK, Ausemus ER, Stakman EC, Bailey CH, Wilson HK, Bamberg RH, Markley MC, Crim RF, Levine MN (1936) Thatcher wheat. Minn Agric Res Stn Bull 325
Jin Y, Singh RP (2006) Resistance in U.S. wheat to recent eastern African isolates of Puccinia graminis f. sp. tritici with virulence to resistance gene Sr31. Plant Dis 90:476–480
Kearsey MJ (1998) The principles of QTL analysis (a minimal mathematics approach). J Exp Bot 49:1619–1623
Kerber ER (1983) Suppression of rust resistance in amphidiploids of Triticum. In: Sakamoto S (ed) Proc 6th Int Wheat Genet Symp, Plant Germplasm Institute, Kyoto University, Japan, pp 813–817
Kerber ER, Aung T (1999) Leaf rust resistance gene Lr34 associated with nonsuppression of stem rust resistance in the wheat cultivar Canthatch. Phytopathology 89:518–521
Knott DR (1999) The inheritance of stem rust resistance in Thatcher wheat. Can J Plant Sci 80:53–63
Knott DR (2001) The relationship between seedling and field resistance to two races of stem rust in Thatcher wheat. Can J Plant Sci 81:415–418
Kolmer JA (1996) Genetics of resistance to wheat leaf rust. Annu Rev Phytopathol 34:435–455
Kolmer JA (2001) Early research on the genetics of Puccinia graminis and stem resistance in wheat in Canada and the United States. In: Campbell CL, Peterson P (eds) Wheat stem rust, from ancient enemy to modern foe. APS Press, St. Paul, MN, USA
Kolmer JA, Dyck PL, Roelfs AP (1991) An appraisal of stem and leaf Rrust resistance in North American hard red spring wheats and the probability of multiple mutations to virulence in populations of cereal rust fungi. Phytopathology 81:237–239
Litt M, Hauge X, Sharma V (1993) Shadow bands seen when typing polymorphic dinucleotide repeats: some causes and cures. BioTechniques 15:280–284
Liu JQ, Kolmer JA (1998) Genetics of stem rust resistance in wheat cvs. Pasqua and AC Taber. Phytopatholology 88:171–176
Long DL, Leonard KJ, Hughes ME (2002) Virulence of Puccinia triticina on wheat in the United States in 1999. Plant Dis 86:15–19
Nazareno NRX, Roelfs AP (1981) Adult plant resistance of Thatcher wheat to stem rust. Phytopathology 71:181–185
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500
Riede CR, Anderson JA (1996) Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci 36:905–909
Roelfs AP, Martens JW (1988) An international system of nomenclature for Puccinia graminis f. sp. tritici. Phytopathology 78:526–533
Samuels M (1999) Statistics for the life sciences, 2nd ed. Prentice Hall, Upper Saddle River, NJ, USA
Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560
Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735
Stakman EC, Levine MN (1922) The determination of biologic forms of Puccinia graminis on Triticum spp. Minn Agric Exp Sta Tech Bull 8
Wanyera R, Kinyua MG, Jin Y, Singh R (2006) The spread of Puccinia graminis f. sp. tritici with virulence on Sr31 in Eastern Africa. Plant Dis 90:113
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Vanegas, C.D.G., Garvin, D.F. & Kolmer, J.A. Genetics of stem rust resistance in the spring wheat cultivar Thatcher and the enhancement of stem rust resistance by Lr34 . Euphytica 159, 391–401 (2008). https://doi.org/10.1007/s10681-007-9541-0
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DOI: https://doi.org/10.1007/s10681-007-9541-0