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Genetics and mapping of seedling resistance to Ug99 stem rust in winter wheat cultivar Triumph 64 and differentiation of SrTmp, SrCad, and Sr42

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Resistance to Ug99 stem rust in Triumph 64 was conferred by SrTmp on chromosome arm 6DS and was mapped to the same position as SrCad and Sr42 , however, the three genes show functional differences.

Abstract

Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is an important disease of wheat that can be controlled by effective stem rust resistance (Sr) genes. The emergence of virulent Pgt races in Africa, namely Ug99 and its variants, has stimulated the search for new Sr genes and genetic characterization of known sources of resistance. Triumph 64 is a winter wheat cultivar that carries gene SrTmp, which confers resistance to Ug99. The goals of this study were to genetically map SrTmp and examine its relationship with other Sr genes occupying a similar chromosome location. A doubled haploid (DH) population from the cross LMPG-6S/Triumph 64 was inoculated with Ug99 at the seedling stage. A single gene conditioning resistance to Ug99 segregated in the population. Genetic mapping with SSR markers placed SrTmp on chromosome arm 6DS in a region similar to SrCad and Sr42. SNP markers developed for SrCad were used to further map SrTmp and were also added to a genetic map of Sr42 using a DH population (LMPG-6S/Norin 40). Three SNP markers that co-segregated with SrTmp also co-segregated with SrCad and Sr42. The SNP markers showed no difference in the map locations of SrTmp, SrCad, and Sr42. Multi-race testing with DH lines from the Triumph 64 and Norin 40 populations and a recombinant inbred-line population from the cross LMPG-6S/AC Cadillac showed that SrTmp, SrCad, and Sr42 confer different spectra of resistance. Markers closely linked to SrTmp are suitable for marker-assisted breeding and germplasm development.

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Acknowledgments

The authors thank Taye Zegeye, Mira Popovic, Ghassan Mardli, Leslie Bezte, Tobi Malasiuk and Elaine Martineau for excellent technical assistance. We are grateful for the financial support from Agriculture and Agri-Food Canada Growing Forward, AAFC Agriflex project 2521, AAFC A-base project J-000006, the Canadian Wheat Alliance, and as part of CTAG and CTAG2, Genome Prairie projects funded by Genome Canada, Saskatchewan Ministry of Agriculture, and Western Grain Research Foundation. M. Rouse also acknowledges the support from USDA-ARS appropriated project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, and the Durable Rust Resistance in Wheat project. Based upon research contributions from the National Research Council of Canada, this publication was given the Contribution Number NRCC# 56224.

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100 Morden, Manitoba, R6M 1Y5, Canada

    Colin W. Hiebert, Mulualem T. Kassa, Curt A. McCartney & Frank M. You

  2. National Research Council, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

    Mulualem T. Kassa & Pierre Fobert

  3. United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Cereal Disease Laboratory, University of Minnesota Department of Plant Pathology, 1551 Lindig Street, St. Paul, MN, USA

    Matthew N. Rouse

  4. Agriculture and Agri-Food Canada, Brandon Research and Development Centre, Brandon, MB R7A 5Y3, Canada

    Tom G. Fetch

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  1. Colin W. Hiebert
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Correspondence to Colin W. Hiebert.

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Communicated by E. Lagudah.

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Hiebert, C.W., Kassa, M.T., McCartney, C.A. et al. Genetics and mapping of seedling resistance to Ug99 stem rust in winter wheat cultivar Triumph 64 and differentiation of SrTmp, SrCad, and Sr42 . Theor Appl Genet 129, 2171–2177 (2016). https://doi.org/10.1007/s00122-016-2765-4

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