Skip to main content
SpringerLink
Log in

An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Adult plant resistance (APR) to leaf rust and stripe rust derived from the wheat (Triticum aestivum L.) line PI250413 was previously identified in RL6077 (=Thatcher*6/PI250413). The leaf rust resistance gene in RL6077 is phenotypically similar to Lr34 which is located on chromosome 7D. It was previously hypothesized that the gene in RL6077 could be Lr34 translocated to another chromosome. Hybrids between RL6077 and Thatcher and between RL6077 and 7DS and 7DL ditelocentric stocks were examined for first meiotic metaphase pairing. RL6077 formed chain quadrivalents and trivalents relative to Thatcher and Chinese Spring; however both 7D telocentrics paired only as heteromorphic bivalents and never with the multivalents. Thus, chromosome 7D is not involved in any translocation carried by RL6077. A genome-wide scan of SSR markers detected an introgression from chromosome 4D of PI250413 transferred to RL6077 through five cycles of backcrossing to Thatcher. Haplotype analysis of lines from crosses of Thatcher × RL6077 and RL6058 (Thatcher*6/PI58548) × RL6077 showed highly significant associations between introgressed markers (including SSR marker cfd71) and leaf rust resistance. In a separate RL6077-derived population, APR to stripe rust was also tightly linked with cfd71 on chromosome 4DL. An allele survey of linked SSR markers cfd71 and cfd23 on a set of 247 wheat lines from diverse origins indicated that these markers can be used to select for the donor segment in most wheat backgrounds. Comparison of RL6077 with Thatcher in field trials showed no effect of the APR gene on important agronomic or quality traits. Since no other known Lr genes exist on chromosome 4DL, the APR gene in RL6077 has been assigned the name Lr67.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agarwal S, Saini RG (2009) Undescribed wheat gene for partial leaf rust and stripe rust resistance from Thatcher derivatives RL6058 and 90RN2491 carrying Lr34. J Appl Genet 50:199–204

    CAS  PubMed  Google Scholar 

  • American Association of Cereal Chemists (2000) Approved methods of the AACC, 10th edn. The Association, St. Paul

    Google Scholar 

  • Dyck PL (1987) The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469

    Google Scholar 

  • Dyck PL (1993) The inheritance of leaf rust and stem rust resistance in ‘Roblin’ wheat. Genome 36:289–293

    Article  CAS  PubMed  Google Scholar 

  • Dyck PL, Samborski DJ (1979) Adult-plant leaf rust resistance in PI 250413, an introduction of common wheat. Can J Plant Sci 59:329–332

    Article  Google Scholar 

  • Dyck PL, Kerber ER, Aung T (1994) An interchromosomal reciprocal translocation in wheat involving wheat leaf rust resistance gene Lr34. Genome 37:556–559

    Article  CAS  PubMed  Google Scholar 

  • Hayden MJ, Nguyen TM, Waterman A, McMichael GL, Chalmers KJ (2008) Application of multiplex-ready PCR for fluorescence-based SSR genotyping in barley and wheat. Mol Breed 21:271–281

    Article  CAS  Google Scholar 

  • Kerber ER, Aung T (1999) Leaf rust resistance gene Lr34 associated with nonsuppression of stem rust resistance in the wheat cultivar Canthatch. Phytopathology 89:518–521

    Article  CAS  PubMed  Google Scholar 

  • Kolmer JA, Singh RP, Garvin DF, Viccars L, William HM, Huerta-Espino J, Ogbonnaya FC, Raman H, Orford S, Bariana HS, Lagudah ES (2008) Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci 48:1841–1852

    Article  CAS  Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh R, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC Transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363

    Article  CAS  PubMed  Google Scholar 

  • Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898

    Article  CAS  PubMed  Google Scholar 

  • Lillemo M, Singh RP, Huerta-Espino J, Chen XM, He ZH, Brown JKM (2007) Leaf rust resistance gene Lr34 is involved in powdery mildew resistance of CIMMYT bread wheat line Saar. In: Buck HT et al (eds) Wheat production in stressed environments. Springer, The Netherlands, pp 97–102

    Chapter  Google Scholar 

  • Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen X, He Z, Bjørnstad A (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166

    Article  CAS  PubMed  Google Scholar 

  • McCallum BD, Seto-Goh P (2003) Physiologic specialization of wheat leaf rust (Puccinia triticina) in Canada in 2000. Can J Plant Pathol 25:91–97

    Article  Google Scholar 

  • McCartney CA, Somers DJ, Humphreys DG, Lukow O, Ames N, Noll J, Cloutier S, McCallum BD (2005) Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross RL4452 × ‘AC Domain’. Genome 48:870–883

    CAS  PubMed  Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, East Melbourne

    Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  CAS  PubMed  Google Scholar 

  • Pallotta MA, Warner P, Fox RL, Kuchel H, Jefferies SJ, Langridge P (2003) Marker assisted wheat breeding in the southern region of Australia. In: Pogna NE, Romano M, Pogna EA, Galerio G (eds) Proceedings of 10th international wheat genetics symposium. Institutio Sperimentale per la Cerealcoltura, Rome, pp 789–791

    Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow-rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508

    Article  CAS  PubMed  Google Scholar 

  • Samborski DJ (1985) Wheat leaf rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, vol 2. Academic Press, Orlando, pp 39–59

    Google Scholar 

  • Sears ER (1953) Nullisomic analysis in common wheat. Am Nat 87:245–252

    Article  Google Scholar 

  • Singh RP (1992a) Genetic association of leaf rust resistance gene Lr34 with adult-plant resistance to stripe rust in bread wheat. Phytopathology 82:835–838

    Article  Google Scholar 

  • Singh RP (1992b) Genetic association between gene Lr34 for leaf rust resistance and leaf tip necrosis in bread wheats. Crop Sci 32:874–878

    Article  Google Scholar 

  • Singh RP, Mujeeb-Kazi A, Huerta-Espino J (1998) Lr46: a gene conferring slow-rusting resistance to leaf rust in wheat. Phytopatholgy 88:890–894

    Article  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  CAS  PubMed  Google Scholar 

  • Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance is associated with durable leaf rust and stripe rust resistance genes Lr34/Yr18 and maps to a single locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735

    Article  CAS  PubMed  Google Scholar 

  • Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet 116:481–490

    Article  CAS  PubMed  Google Scholar 

  • Vanegas CDG, Garvin DF, Kolmer JA (2008) Genetics of stem rust resistance in the spring wheat cultivar Thatcher and the enhancement of stem rust resistance by Lr34. Euphytica 159:391–401

    Article  CAS  Google Scholar 

  • William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93:153–159

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We acknowledge the excellent technical assistance of Pat Seto-Goh, Mira Popovic, Erica Riedel and Denis Green in Canada and Jessica Hyles and Keshab Kandel in Australia. We also thank the Grains Research and Development Corportion (GRDC), Australia for financial support.

Author information

Authors and Affiliations

  1. Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd, Winnipeg, MB, R3T 2M9, Canada

    Colin W. Hiebert, Julian B. Thomas, Brent D. McCallum & D. Gavin Humphreys

  2. Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada, P.O. Box 1030, Airport Rd, Swift Current, SK, S9H 3X2, Canada

    Ronald M. DePauw

  3. Department of Primary Industries, Victorian AgriBioscience Centre, La Trobe Research and Development Park, Bundoora, VIC, 3082, Australia

    Matthew J. Hayden

  4. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Rohit Mago, Wendelin Schnippenkoetter & Wolfgang Spielmeyer

Authors
  1. Colin W. Hiebert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Julian B. Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brent D. McCallum
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Gavin Humphreys
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ronald M. DePauw
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Matthew J. Hayden
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rohit Mago
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wendelin Schnippenkoetter
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Wolfgang Spielmeyer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Colin W. Hiebert.

Additional information

Communicated by B. Friebe.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table S1 (DOC 236 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hiebert, C.W., Thomas, J.B., McCallum, B.D. et al. An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor Appl Genet 121, 1083–1091 (2010). https://doi.org/10.1007/s00122-010-1373-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-010-1373-y

Keywords

Search

Navigation