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Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species

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Abstract

The first microsatellite linkage map of Ae. speltoides Tausch (2n = 2x = 14, SS), which is a wild species with a genome closely related to the B and G genomes of polyploid wheats, was developed based on two F2 mapping populations using microsatellite (SSR) markers from Ae. speltoides, wheat genomic SSRs (g-SSRs) and EST-derived SSRs. A total of 144 different microsatellite loci were mapped in the Ae. speltoides genome. The transferability of the SSRs markers between the related S, B, and G genomes allowed possible integration of new markers into the T. timopheevii G genome chromosomal maps and map-based comparisons. Thirty-one new microsatellite loci assigned to the genetic framework of the T. timopheevii G genome maps were composed of wheat g-SSR (genomic SSR) markers. Most of the used Ae. speltoides SSRs were mapped onto chromosomes of the G genome supporting a close relationship between the G and S genomes. Comparative microsatellite mapping of the S, B, and G genomes demonstrated colinearity between the chromosomes within homoeologous groups, except for intergenomic T6AtS.1G, T4AL.5AL.7BS translocations. A translocation between chromosomes 2 and 6 that is present in the T. aestivum B genome was found in neither Ae. speltoides nor in T. timopheevii. Although the marker order was generally conserved among the B, S, and G genomes, the total length of the Ae. speltoides chromosomal maps and the genetic distances between homoeologous loci located in the proximal regions of the S genome chromosomes were reduced compared with the B, and G genome chromosomes.

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Acknowledgments

The authors would like to thank Prof. T.I. Aksenovich, IC&G SB RAS, Novosibirsk, Russia, and the anonymous referee for helpful comments and suggestions on the paper. This study was supported, in part, by an integration project (no. 129) of the Siberian Branch of the Russian Academy of Sciences, the Ministry of Education and Science of the Russian Federation (a state contract no. P409) and Russian Foundation for Basic Research under grant 10-04-01458-a.

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  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentieva Ave. 10, Novosibirsk, 630090, Russia

    O. Dobrovolskaya & E. Salina

  2. UMR INRA-UBP Génétique, Diversité et Ecophysiologie des Céréales, 234 Avenue du Brézet, Domaine de Crouël, 63100, Clermont-Ferrand Cedex 2, France

    C. Boeuf, J. Salse, C. Pont, P. Sourdille & M. Bernard

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  1. O. Dobrovolskaya
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  2. C. Boeuf
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  3. J. Salse
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  4. C. Pont
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  5. P. Sourdille
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  6. M. Bernard
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  7. E. Salina
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Correspondence to O. Dobrovolskaya.

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Communicated by J. Reif.

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Dobrovolskaya, O., Boeuf, C., Salse, J. et al. Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species. Theor Appl Genet 123, 1145–1157 (2011). https://doi.org/10.1007/s00122-011-1655-z

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