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Major-effect QTLs for stem and foliage resistance to late blight in the wild potato relatives Solanum sparsipilum and S. spegazzinii are mapped to chromosome X

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Abstract

To find out new resistance sources to late blight in the wild germplasm for potato breeding, we examined the polygenic resistance of Solanum sparsipilum and S. spegazzinii by a quantitative trait locus (QTL) analysis. We performed stem and foliage tests under controlled conditions in two diploid mapping progenies. Four traits were selected for QTL detection. A total of 30 QTLs were mapped, with a large-effect QTL region on chromosome X detected in both potato relatives. The mapping of literature-derived markers highlighted colinearities with published late blight QTLs or R-genes. Results showed (a) the resistance potential of S. sparsipilum and S. spegazzinii for late blight control, and (b) the efficacy of the stem test as a complement to the foliage test to break down the complex late blight resistance into elementary components. The relationships of late blight resistance QTLs with R-genes and maturity QTLs are discussed.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

CAPS:

Cleaved amplified polymorphic sequence

DPI:

Days post-inoculation

PCA:

Principal component analysis

QTL:

Quantitative trait locus

rAUDPC:

Time-relative area under the disease progress curve

RFLP:

Restriction fragment length polymorphism

RGA:

Resistance gene analogue

SCAR:

Sequence characterized amplified region

SD:

Standard deviation

SSR:

Simple sequence repeat

STS:

Sequence tagged site

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Acknowledgments

We gratefully thank J.-P. Dantec for his participation in the phenotyping work, M. Ardisson, K. Chevalier, N. Lama, S. Mauger, C. Poncet and P. Signoret for their contribution to the marker genotyping work, D. Andrivon and R. Corbière for having provided the NII.72.10 P. infestans isolate, F. Fabre for his help in programming the statistical tests with the R software, and S. Mallard for her valuable comments on the manuscript. At last but not the least, we are indebted to anonymous referees for insightful comments on our manuscript. This work was financially supported by the European BIOEXPLOIT project FOOD CT2005-513959, the JOINPOT INRA project devoted to SSR genotyping performed on the Clermont-Ferrand genotyping platform (France) and the French Agriculture and Fishery Ministry in the frame of the Contract 05-30-PdTerre devoted to the development of marker-assisted selection in potato breeding. S. Danan received a PhD fellowship funded by the European BIOEXPLOIT project FOOD CT 2005-513959.

Author information

Author notes

  1. Jean-Denis Moal

    Present address: Comité Nord, Station de recherche de Bretteville, 76110, Bretteville du Grand Caux, France

Authors and Affiliations

  1. INRA, UR 1052 GAFL Génétique et Amélioration des Fruits et Légumes, BP 94, 84140, Montfavet, France

    Sarah Danan, Bernard Caromel & Véronique Lefebvre

  2. INRA, UMR 118 APBV Amélioration des Plantes et Biotechnologies Végétales, 29260, Ploudaniel, France

    Jean-Eric Chauvin, Jean-Denis Moal & Roland Pellé

Authors
  1. Sarah Danan
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  2. Jean-Eric Chauvin
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  3. Bernard Caromel
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  4. Jean-Denis Moal
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  5. Roland Pellé
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  6. Véronique Lefebvre
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Corresponding author

Correspondence to Véronique Lefebvre.

Additional information

Communicated by T. Luebberstedt.

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Danan, S., Chauvin, JE., Caromel, B. et al. Major-effect QTLs for stem and foliage resistance to late blight in the wild potato relatives Solanum sparsipilum and S. spegazzinii are mapped to chromosome X. Theor Appl Genet 119, 705–719 (2009). https://doi.org/10.1007/s00122-009-1081-7

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  • DOI: https://doi.org/10.1007/s00122-009-1081-7

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