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QTL analysis of late blight resistance in a diploid potato family of Solanum phureja × S. stenotomum

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Abstract

Field resistance to Phytophthora infestans (Mont.) de Bary, the causal agent of late blight in potatoes, has been characterized in a potato segregating family of 230 full-sib progenies derived from a cross between two hybrid Solanum phureja × S. stenotomum clones. The distribution of area under the disease progress curve values, measured in different years and locations, was consistent with the inheritance of multigenic resistance. Relatively high levels of resistance and transgressive segregations were also observed within this family. A genetic linkage map of this population was constructed with the intent of mapping quantitative trait loci (QTLs) associated with this late blight field resistance. A total of 132 clones from this family were genotyped based on 162 restriction fragment length polymorphism (RFLP) markers. The genome coverage by the map (855.2 cM) is estimated to be at least 70% and includes 112 segregating RFLP markers and two phenotypic markers, with an average distance of 7.7 cM between two markers. Two methods were employed to determine trait–marker association, the non-parametric Kruskal–Wallis test and interval mapping analysis. Three major QTLs were detected on linkage group III, V, and XI, explaining 23, 17, and 10%, respectively, of the total phenotypic variation. The present study revealed the presence of potentially new genetic loci in this diploid potato family contributing to general resistance against late blight. The identification of these QTLs represents the first step toward their introgression into cultivated tetraploid potato cultivars through marker-assisted selection.

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References

  • Black W, Mastenbroek C, Mills WR, Peterson LC (1953) A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives. Euphytica 2:173–178

    Article  Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    CAS  PubMed  Google Scholar 

  • Collins A, Milbourne D, Ramsay L, Meyer R, Chatot-Balandras C, Oberhagemann P, De Jong W, Gebhardt C, Bonnel E, Waugh R (1999) QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour. Mol Breed 5:387–398

    Article  CAS  Google Scholar 

  • Costanzo S, Christ BJ, Haynes KG (2004) Late blight resistance in a diploid full-sib potato family. Plant Breed 123:377–381

    Google Scholar 

  • De Vicente MC, Tanksley SD (1993) QTL analysis of transgressive segregation in an interspecific tomato cross. Genetics 134:585–596

    CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • El-Kharbotly A, Leonards-Schippers C, Huigen DJ, Jacobsen E, Pereira A, Stiekema WJ, Salamini F, Gebhardt C (1994) Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242:749–754

    Article  CAS  PubMed  Google Scholar 

  • El-Kharbotly A, Palomino-Sanchez C, Salamini F, Jacobsen E, Gebhardt C (1996) R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theor Appl Genet 92:880–884

    Article  CAS  Google Scholar 

  • Ewing EE, Simko I, Smart CD, Bonierbale MW, Mizubuti ESG, May GD, Fry WE (2000) Genetic mapping from field tests of qualitative and quantitative resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii. Mol Breed 6:25–36

    Article  CAS  Google Scholar 

  • Fry WE, Goodwin SB (1997) Re-emergence of potato and tomato late blight in the United States. Plant Dis 81:1349–1357

    Google Scholar 

  • Gebhardt C, Valkonen JPT (2001) Organization of genes controlling disease resistance in the potato genome. Ann Rev Phytopathol 39:79–102

    Article  CAS  Google Scholar 

  • Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thomson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57

    Article  Google Scholar 

  • Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E (1993) Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato. Theor Appl Genet 85:541–544

    Google Scholar 

  • Gebhardt C, Eberle B, Leonards-Schippers C, Walkemeier B, Salamini F (1995) Isolation, characterization and RFLP linkage mapping of a DNA repeat family of Solanum spegazzinii by which chromosome ends can be localized on the genetic map of potato. Genet Res 65:1–10

    CAS  PubMed  Google Scholar 

  • Gebhardt C, Ritter E, Salamini F (2001) RFLP map of the potato. In: Phillips R, Vasil I (eds) DNAbased markers in plants, 2nd edn. Advances in cellular and molecular biology of plants, vol 6. Kluwer, Dordrecht, pp 512

  • Ghislain M, Trognitz B, Herrera MR, Solis J, Casallo G, Vasquez C, Hurtado O, Castillo R, Portal L, Orrillo M (2001) Genetic loci associated with field resistance to late blight in offspring of Solanum phureja and S. tuberosum grown under short-day conditions. Theor Appl Genet 103:433–442

    Google Scholar 

  • Goodwin SB, Sujkowski LS, Fry WE (1995) Rapid evolution of pathogenicity within clonal lineages of the potato late blight disease fungus. Phytopathol 85:669–676

    Google Scholar 

  • Haynes KG, Christ BJ (1999) Heritability of resistance to foliar late blight in a diploid hybrid potato population of Solanum phureja × Solanum stenotomum. Plant Breed 118:431–434

    Google Scholar 

  • Hodgson WA (1961) Laboratory testing of the potato for partial resistance to Phytophthora infestans. Am Potato J 38:259–264

    Google Scholar 

  • Hollander M, Wolfe D (1973) Nonparametric statistical methods. Wiley series in probability and mathematical statistics. Wiley, New York

    Google Scholar 

  • Jacobs JME, van Eck HJ, Horsman K, Arens P, Verkerk-Bakker B, Jacobsen E, Pereira A, Stiekema WJ (1996) Mapping of resistance to the potato cyst nematode Globodera rostochiensis from the wild potato species Solanum vernei. Mol Breed 2:51–60

    Article  CAS  Google Scholar 

  • Jansen RC (1993) Interval mapping of multiple quantitative trait loci. Genetics 135:205–211

    CAS  PubMed  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Kuhl JC, Hanneman Jr RE, Havey MJ (2001) Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Mol Genet Genomics 265:977–985

    Article  CAS  PubMed  Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Lehmann EL and D’Abrera HJM (1975) Nonparametrics: statistical methods based on ranks. Holden–Day series in probability and statistics. Holden–Day, San Francisco

    Google Scholar 

  • Leister D, Ballvora A, Salamini F, Gebhardt C (1996) A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nature Genetics 14:421–429

    Google Scholar 

  • Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in allogamus plant species. Genetics 137:67–77

    CAS  PubMed  Google Scholar 

  • Liu Z, Christ BJ, Haynes KG (1998a) Detached leaf evaluation of late blight resistance in twelve clones from a diploid Solanum phureja × S. stenotomum hybrid population. Phytopathology 88:S55

    Google Scholar 

  • Liu Z, Christ BJ, Haynes KG (1998b) Development of molecular markers for tagging late blight resistance (LBR) genes of potato. Phytopathology 88:S55

    Google Scholar 

  • Malcolmson JF, Black W (1966) New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15:199–203

    Article  Google Scholar 

  • Meyer RC, Milbourne D, Hackett CA, Bradshaw JE, McNichol JW, Waugh R (1998) Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans). Mol Gen Genet 259:150–160

    Article  CAS  PubMed  Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2000) Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 101:697–704

    Google Scholar 

  • Oberhagemann P, Chatot-Balandras C, Schäfer-Pregl R, Wegener D, Palomino C, Salamini F, Bonnel E, Gebhardt C (1999) A genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection. Mol Breed 5:399–415

    Article  CAS  Google Scholar 

  • Ooijen JW van, Voorrips R (2001) JoinMap, software for the calculation of genetic linkage maps. Plant Research International, Wageningen

  • Ooijen JW van, Boer M, Jansen R, Maliepaard C (2002) MapQTL, software for the calculation of QTL positions on genetic maps. Plant Research International, Wageningen

  • Pineda O, Bonierbale MW, Plaisted RL, Brodie BB, Tanksley SD (1993) Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematode (Globodera rostochiensis). Genome 36:152–156

    CAS  Google Scholar 

  • Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Reptr 15:8–15

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Sandbrink JM, Colon LT, Wolters PJCC, Stiekema WJ (2000) Two related genotypes of Solanum microdontum carry different segregating alleles for field resistance to Phytophthora infestans. Mol Breed 6:215–225

    Article  CAS  Google Scholar 

  • Shaner G, Finney RE (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67:1051–1056

    CAS  Google Scholar 

  • Simko I (2002) Comparative analysis of quantitative trait loci for foliage resistance to Phytophthora infestans in tuber-bearing Solanum species. Am J Potato Res 79:125–132

    CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    CAS  Google Scholar 

  • Tanksley SD, Ganal MW, Prince, JP, de Vicente MC, Bonierbale MW, Broun, P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L., Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Umaerus V, Umaerus M, Erjefalt L, Nilsson BA (1983) Control of Phytophthora infestans by host resistance: problem and progress. In: Erwin DC, Bartnicki-Garcia S, Tsao PH (eds) Phytophthora: its biology, taxonomy, ecology, and pathology. American Phytopathological Society, St. Paul, pp 315–326

    Google Scholar 

  • Visker MH, Keizer LC, Van Eck HJ, Jacobsen E, Colon LT, Struik P (2003) Can the QTL for late blight resistance on potato chromosome 5 be attributed to foliage maturity type? Theor Appl Genet 106:317–325

    Google Scholar 

  • Voorrips R (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are grateful to Dr. Christiane Gebhardt, Max Planck Institute for Plant Breeding Research, Cologne, Germany, and Dr. Steven D. Tanksley, Department of Plant Biology, Cornell University, Ithaca, N.Y., USA, for providing the RFLP markers used in the present study. This research was supported in part by The Agricultural Research Funds administered by The Pennsylvania Department of Agriculture (no. ME449305); the USDA (CSREES) Special Research Grants Program Potato Research; and The College of Agricultural Sciences, The Pennsylvania State University.

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  1. S. Costanzo & I. Simko

    Present address: Vegetable Laboratory, USDA–ARS–PSI, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA

Authors and Affiliations

  1. Department of Plant Pathology, The Pennsylvania State University, University Park, PA, 16802, USA

    S. Costanzo & B. J. Christ

  2. Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD, 20742

    I. Simko

  3. Vegetable Laboratory, USDA–ARS–PSI, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA

    K. G. Haynes

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  1. S. Costanzo
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Correspondence to B. J. Christ.

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Communicated by F. Salamini

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Costanzo, S., Simko, I., Christ, B.J. et al. QTL analysis of late blight resistance in a diploid potato family of Solanum phureja × S. stenotomum. Theor Appl Genet 111, 609–617 (2005). https://doi.org/10.1007/s00122-005-2053-1

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  • DOI: https://doi.org/10.1007/s00122-005-2053-1

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