Skip to main content
SpringerLink
Log in

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Allele mining facilitates the discovery of novel resistance (R) genes that can be used in breeding programs and sheds light on the evolution of R genes. Here we focus on two R genes, Rpi-blb1 and Rpi-blb2, originally derived from Solanum bulbocastanum. The Rpi-blb1 gene is part of a cluster of four paralogues and is flanked by RGA1-blb and RGA3-blb. Highly conserved RGA1-blb homologues were discovered in all the tested tuber-bearing (TB) and non-tuber-bearing (NTB) Solanum species, suggesting RGA1-blb was present before the divergence of TB and NTB Solanum species. The frequency of the RGA3-blb gene was much lower. Interestingly, highly conserved Rpi-blb1 homologues were discovered not only in S. bulbocastanum but also in Solanum stoloniferum that is part of the series Longipedicellata. Resistance assays and genetic analyses in several F1 populations derived from the relevant late blight resistant parental genotypes harbouring the conserved Rpi-blb1 homologues, indicated the presence of four dominant R genes, designated as Rpi-sto1, Rpi-plt1, Rpi-pta1 and Rpi-pta2. Furthermore, Rpi-sto1 and Rpi-plt1 resided at the same position on chromosome VIII as Rpi-blb1 in S. bulbocastanum. Segregation data also indicated that an additional unknown late blight resistance gene was present in three populations. In contrast to Rpi-blb1, no homologues of Rpi-blb2 were detected in any material examined. Hypotheses are proposed to explain the presence of conserved Rpi-blb1 homologues in S. stoloniferum. The discovery of conserved homologues of Rpi-blb1 in EBN 2 tetraploid species offers the possibility to more easily transfer the late blight resistance genes to potato varieties by classical breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ballvora A, Ercolano MR, Weiss J, Meksem K, Bormann CA, Oberhagemann P, Salamini F, Gebhardt C (2002) The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant J 30:361–371

    Article  PubMed  CAS  Google Scholar 

  • Black W, Mastenbroek C, Mills WR, Peterson LC (1953) A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives. Euphytica 2:173–179

    Article  Google Scholar 

  • Colton LM, Groza HI, Wielgus SM, Jiang J (2006) Marker-assisted selection for the broad-spectrum potato late blight resistance conferred by gene RB derived from a wild potato species. Crop Sci 46:589–594

    Article  CAS  Google Scholar 

  • Ewing EE, Simko I, Smart CD, Bonierbale MW, Izubuti ESG, May GD, Fry WE (2000) Genetic mapping from field tests of qualitative and quantitative resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii. Mol Breed 6:25–36

    Article  CAS  Google Scholar 

  • Flier WG van den Bosch GBM, Turkensteen LJ (2003) Stability of partial resistance in potato cultivars exposed to aggressive strains of Phytophthora infestans. Plant Path 52:326–337

    Article  Google Scholar 

  • Ghislain M, Trognitz B, Herrera M, Solis J, Casallo G, Vasquez C, Hurtado O, Castillo R, Portal L, Orrillo M (2001) Genetic loci associated with field resistance to late blight in offspring of S. phureja and S. tuberosum grown under short-day conditions. Theor Appl Genet 103:433–442

    Article  CAS  Google Scholar 

  • GILB (2004) Global initiative on late blight, http://www.cipotato.org/gilb/

  • Hawkes JG (1990) The Potato-Evolution, biodiversity and genetic resources, Belhaven Press, London, UK

    Google Scholar 

  • Helgeson JP, Pohlman JD, Austin S, Haberlach GT, Wielgus SM, Ronis D, Zambolim L, Tooley P, McGrath JM, James RV, Stevenson WR (1998) Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742

    Article  Google Scholar 

  • Hermsen JGTh, Ramanna MS (1973) Double-bridge hybrids of Solanum bulbocastanum and cultivars of Solanum tuberosum. Euphytica 22:457–466

    Article  Google Scholar 

  • Hosaka K, Ogihara Y, Matsubayashi M, Tsunewaki K (1984) Phlogenetic relationship between the tuberous Solanum species as revealed by restriction endonuclease analysis of chloroplast DNA. Jpn J Genet 59:349–369

    Article  CAS  Google Scholar 

  • Huang S, Vleeshouwers VGAA, Werij JS, Hutten RCB, van Eck HJ, Visser RGF, Jacobsen E (2004) The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Mol Plant Microbe Interact 17:428–435

    Article  PubMed  CAS  Google Scholar 

  • Huang S, van der Vossen E, Kuang H, Vleeshouwers VGAA, Zhang N, Borm TJA., van Eck HJ, Baker B, Jacobsen E, Visser RGF (2005) Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant J 42:251–261

    Article  PubMed  CAS  Google Scholar 

  • Hutten RCB, van Berloo R (2001) An online potato pedigree database. URL: http://www.dpw.wau.nl/pv/query.asp

  • Jackson SA, Hanneman RE Jr (1999) Crossability between cultivated and wild tuber-and non-tuber-bearing Solanums. Euphytica 109:51–67

    Article  Google Scholar 

  • Janssen GJW, van Norel A, Verkerk-Bakker B, Janssen R (1995) Resistance to Meloidogyne chitwoodi, M. fallax and M. hapla in wild tuber-bearing Solanum spp. Euphytica 92:287–294

    Article  Google Scholar 

  • Kuhl JC, Hanneman RE, Havey MJ (2001) Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Mol Genet Genomics 265:977–985

    Article  PubMed  CAS  Google Scholar 

  • Matsubayashi M (1991) Phylogenetic relationships in the potato and its related species. In: Tsuchiya T, Gupta PK (eds) Chromosome engineering in plants: genetics, breeding, evolution. Part B. Elsevier, Amsterdam, The Netherlands, pp 93–118

    Google Scholar 

  • Malcolmson JF, Black W (1966) New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15:199–203

    Article  Google Scholar 

  • Michelmore RW, Meyers BC (1998) Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process. Genome Res 8:1113–1130

    PubMed  CAS  Google Scholar 

  • Meyers BC, Kozik A, Griego A, Kuang H, Michelmore RW (2003) Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell 15:809–834

    Article  PubMed  CAS  Google Scholar 

  • Milligan SB, Bodeau J, Yaghoobi J, Kaloshian I, Zabel P, Williamson VM (1998) The root-knot nematode resistance gene Mi from tomato is a member of the leucine zipper nucleotide binding leucine-rich repeat family of plant genes. Plant Cell 10:1307–1319

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2000) Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 101:697–704

    Article  CAS  Google Scholar 

  • Park T, Vleeshouwers VGAA, Hutten RCB, van Eck HJ, van der Vossen E, Jacobsen E, Visser RGF (2005) High-resolution mapping and analysis of the resistance locus Rpi-abpt against Phytophthora infestans in potato. Mol Breed 16:33–43

    Article  CAS  Google Scholar 

  • Rauscher GM, Smart CD, Simko I, Bonierbale M, Mayton H, Greenland A., Fry WE (2006) Characterization and mapping of Rpi-ber, a novel potato late blight resistance gene from Solanum berthaultii. Theor Appl Genet 112:674–687

    Article  PubMed  CAS  Google Scholar 

  • Rouppe van der Voort J, Lindeman W, Folkertsma R, Hutten RCB, Overmars H, van der Vossen E, Jacobsen E, Bakker J (1998) A QTL for broad-spectrum resistance to cyst nematode species (Globodera spp.) maps to a resistance gene cluster in potato. Theor Appl Genet 96:654–661

    Article  Google Scholar 

  • Sandbrink JM, Colon LT, Wolters PJCC, Stiekema WJ (2000) Two related genotypes of Solanum microdontum carry different segregating alleles for field resistance to Phytophthora infestans. Mol Breed 6:215–225

    Article  CAS  Google Scholar 

  • Sliwka J, Jakuczun H, Lebecka R, Marczewski W, Gebhardt C, Zimnoch-Guzowska E (2006) The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period. Theor Appl Genet 113:685–695

    Article  PubMed  CAS  Google Scholar 

  • Smilde WD, Brigneti G, Jagger L, Perkins S, Jones JDG (2005) Solanum mochiquense chromosome IX carries a novel late blight resistance gene Rpi-moc1. Theor Appl Genet 110:252–258

    Article  PubMed  CAS  Google Scholar 

  • Song J, Bradeen JM, Naess SK, Raasch JA, Wielgus SM, Haberlach GT, Liu J, Kuang H, Austin-Phillips S, Buell CR, Helgeson JP, Jiang J (2003) Gene RB cloned from Solanumbulbocastanum confers broad spectrum resistance to potato late blight. Proc Natl Acad Sci 100:9128–9133

    Article  PubMed  CAS  Google Scholar 

  • Spooner DM, van den Berg RG, Rodrigues A, Bamberg J, Hijmans RJ, Lara-Cabrera S (2004) Wild Potatoes (Solanum Section Petota) of North and Central America. Syst Bot Monogr 68:1–209

    Google Scholar 

  • Spooner DM, van den Berg RG, Miller JT (2001) Species and series boundaries of Solanum series Longipedicellata (Solanaceae) and phenetically similar species in ser. Demissa and ser. Tuberosa: implications for a practical taxonomy of sect. Petota. Am J Bot 88:113–130

    Article  Google Scholar 

  • Stewart CNJ, Via LE (1993) A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques 14:748–750

    PubMed  CAS  Google Scholar 

  • van den Berg RG, Bryan GJ, del Rio A, Spooner DM (2002) Reduction of species in the wild potato Solanum section Petota series Longipedicellata. AFLP, RAPD and chloroplast SSR data. Theor Appl Genet 105:1109–1114

    Article  Google Scholar 

  • van der Vossen E, Sikkema A, Hekkert BL, Gros J, Stevens P, Muskens M, Wouters D, Pereira A, Stiekema W, Allefs S (2003) An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant J 36:867–882

    Article  PubMed  Google Scholar 

  • van der Vossen E, Gros J, Sikkema A, Muskens M, Wouters D, Wolters P, Pereira A, Allefs S (2005) The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato. Plant J 44:208–222

    Article  PubMed  Google Scholar 

  • Vleeshouwers VGAA, van Dooijeweert W, Keizer LCP, Sijpkes L, Govers F, Colon LT (1999) A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation. Eur J Plant Pathol 105:241–250

    Article  Google Scholar 

  • Villamon FG, Spooner DM, Orrillo M, Mihovilovich E, Perez W, Bonierbale M (2005) Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana). Theor Appl Genet 111:1201–1214

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by a grant from the EU (contract No. FOOD-CT-2005-513959 BIOEXPLOIT) for the Bioexploit project and by funding from Plant Research International, Wageningen University and Research Centre.

Author information

Authors and Affiliations

  1. Plant Research International B.V., Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Miqia Wang, Edwin A. G. van der Vossen & Ben Vosman

  2. Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei, 430062, China

    Miqia Wang

  3. Agrico Research, PO Box 40, 8300 AA, Emmeloord, The Netherlands

    Sjefke Allefs

  4. Biosystematics Group, Wageningen University and Research Centre, Generaal Foulkesweg 37, P.O. Box 8010, 6700 ED, Wageningen, The Netherlands

    Ronald G. van den Berg

  5. Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Vivianne G. A. A. Vleeshouwers

Authors
  1. Miqia Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sjefke Allefs
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ronald G. van den Berg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vivianne G. A. A. Vleeshouwers
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Edwin A. G. van der Vossen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ben Vosman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ben Vosman.

Additional information

Communicated by J. E. Bradshaw.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, M., Allefs, S., van den Berg, R.G. et al. Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum . Theor Appl Genet 116, 933–943 (2008). https://doi.org/10.1007/s00122-008-0725-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-008-0725-3

Keywords

Search

Navigation