Abstract
A high level of resistance toGlobodera pallida pathotypes Pa2 and Pa3 exists inSolanum spegazzinii, a wild relative of potato (S. tuberosum ssp.tuberosum). Here we report the mapping of loci involved in quantitatively-inherited nematode resistance with the use of RFLPs. One major locus,Gpa, was mapped on chromosome 5 and two minor loci on chromosomes 4 and 7 ofS. spegazzinii. Additionally, the contribution of the susceptible parent to nematode resistance was determined. TheGpa locus was solely responsible for the high resistance level found in the segregating population. However, the RFLP marker closely linked to this resistance locus showed a distorted segregation, with a shortage of plants having the resistance linked allele. Our results indicate that a prediction of the genetic constitution of a quantitative trait based solely on phenotypic observations can lead to erroneous conclusions.
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Arntzen FK, Van Eeuwijk FA (1992) Variation in resistance level of potato genotype and virulence level of potato cyst nematode populations. Euphytica 62: 135–143
Arntzen FK, Vinke JH, Hoogendoorn J (1993) Inheritance, level and origin of resistance toGlobodera pallida in the potato cultivar Multa, derived fromSolanum tuberosum ssp.andigena CPC 1673. FundAppl Nematol 16: 155–162
Bonierbale MW, Plaisted R, Tanksley SD (1992) Genetic mapping and utilization of quantitative trichome-mediated insect resistance in potato. Neth J Pl Pathol Suppl 2: 211–214
Dale MFB, Phillips MS (1982) An investigation of resistance to the white potato cyst-nematode. J Agric Sci 99: 325–328
Dellaert LMW, Hoekstra R (1987) Resistance to potato cyst nematodes,Globodera spp., in wild and primitiveSolanum species. Potato Res 30: 579–587
Dellaert LMW, Vinke H, Meyer K (1988) The inheritance of resistance to the potato cyst-nematodeGlobodera pallida Pa3 in wild Solanum species with broad spectrum resistance. Euphytica Suppl: 105–116
Dunnet JM (1962) Inheritance of resistance to potato root eelworm in a breeding line stemming fromSolanum multidissectum Hawkes. In: Anonymous (eds), Ann Report Scottish Plant Breed Station 99161:39–46
Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E (1993) Identification of RFLP markers closely linked to theH1 gene conferring resistance toGlobodera rostochiensis in potato. Theor Appl Genet 85: 541–544
Howard HW, Cole CS, Fuller JM (1970) Further sources of resistance toHeterodera rostochiensis Woll. in the andigena potatoes. Euphytica 19: 210–216
Jackson MT, Hawkes JG, Male-Kayiwa BS, Wanyers NWM (1988) The importance of the Bolivian wild potato species in breeding forGlobodera pallida resistance. Plant Breed 101: 261–268
Jones FGW, Carpenter JM, Parrott DM, Stone AR, Trudgill DL (1970) Potato cyst nematode: one species or two? Nature 227: 83–84
Kort J, Ross H, Rumpenhorst HJ, Stone AR (1977) An international scheme for identifying and classifying pathotypes of potato cyst nematodesGlobodera rostochiensis andG. Pallida. Nematologica 23: 333–339
Kreike CM, de Koning JRA, Vinke JH, van Ooijen JW, Gebhardt C, Stiekema WJ (1993) Mapping of loci involved in quantitatively-inherited resistance to the potato cyst-nematodeGlobodera rostochiensis pathotype Ro1. Theor Appl Genet 87:464–470
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199
Leonards-Schippers C, Gieffen W, Salamini F, Gebhardt C (1992) The R1 gene conferring race-specific resistance toPhytophthora infestans on potato is located on chromosome V. Mol Gen Genet 233: 278–283
Parrott DM (1981) Evidence for a gene-for-gene relationship between resistance gene H1 fromSolanum tuberosum ssp.andigena and a gene inGlobodera rostochiensis, and between H2 fromS. multidissectum and a gene inG. pallida. Nematologica 27: 372–384
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335: 721–726
Pineda O, MW Bonierbale, Plaisted RL, Brodie BB, Tanksley SD (1993). Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematodeGlobodera rostochiensis. Genome 36: 152–156.
Ritter E, Debener T, Barone A, Salamini F, Gebhardt C (1991) RFLP mapping on potato chromosomes of two genes controlling extreme resistance to potato virus X (PVX). Mol Gen Genet 27: 81–85
Ross H (1986) Potato breeding — problems and perspectives. Advances in Plant Breeding 13
Stam P (1993) Construction of integrated linkage maps by means of a new computer package: JoinMap. Plant J 3: 739–744
Stone AR (1972)Heterodera pallida n sp. (Nematoda: Heteroderidae), a second species of potato cyst nematode. Nematologica 18: 591–606
Turner SJ (1989) New sources of resistance to potato cyst nematodes in the Commonwealth Potato Collection. Euphytica 42: 145–153
Van Soest LJM, Rumpenhorst HJ, Huijsman CA (1983) Resistance to potato cyst-nematodes in tuber-bearingSolanum species and its geographical distribution. Euphytica 32: 65–74
Van Ooijen JW (1992) Accuracy of mapping quantitative trait loci in autogamous species. Theor Appl Genet 84: 803–811
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Kreike, C.M., de Koning, J.R.A., Vinke, J.H. et al. Quantitatively-inherited resistance toGlobodera pallida is dominated by one major locus inSolanum spegazzinii . Theoret. Appl. Genetics 88, 764–769 (1994). https://doi.org/10.1007/BF01253983
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DOI: https://doi.org/10.1007/BF01253983