Abstract
Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality.
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References
Andersen JR, Lübberstedt T (2003) Functional markers in plants. Trends Plant Sci 8:554–560
Bagge M, Xia XC, Lübberstedt T (2007) Functional markers in wheat. Curr Opin Plant Biol 10:211–216
Bietz JA, Shepherd KW, Wall JS (1975) Single kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem 52:513–532
Butow BJ, Gale KR, Ikea J, Juhász A, Bedö Z, Tamás L, Gianibelli MC (2004) Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor Appl Genet 109:1525–1535
Cassidy BG, Dvorak J, Anderson OD (1998) The wheat low-molecular-weight glutenin genes: characterization of six new genes and progress in understanding gene family structure. Theor Appl Genet 96:743–750
Cornish GB, Burridge PM, Palmer GA, Wrigley CW (1993) Mapping the origins of some HMW and LMW glutenin subunit alleles in Australian germplasm. In: Proceedings of the 43rd Australian cereal chemistry conference, Sydney, 12–16 September 1993, pp 255–260
D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39:321–339
D’Ovidio R, Simeone M, Masci S, Porceddu E (1997) Molecular characterization of an LMW-GS gene located on chromosome 1B and the development of primers specific for the Glu-B3 complex locus in durum wheat. Theor Appl Genet 95:1119–1126
D’Ovidio R, Marchitelli C, Cardelli LE, Porceddu E (1999) Sequence similarity between allelic Glu-B3 genes related to quality properties of durum wheat. Theor Appl Genet 93:455–461
Dupont FM, Vensel W, Encarnacao T, Chan R, Kasarda DD (2004) Similarities of omega gliadin from Triticum urartu to those encoded on chromosome 1A of hexaploid wheat and evidence for their post-translational processing. Theor Appl Genet 108:1299–1308
Eagles HA, Bariana HS, Ogbonnaya FC, Rebetzke GJ, Hollamby GJ, Henry RJ, Henschke PH, Carter M (2001) Implementation of markers in Australian wheat breeding. Aust J Agric Res 52:1349–1356
Gale KR (2005) Diagnostic DNA markers for quality traits in wheat. J Cereal Sci 41:181–192
Gale KR, Ma W, Zhang W, Rampling L, Hill AS, Appels R, Morris P, Morrel M (2001) Simple high-throughput DNA markers for genotyping in wheat. In: Eastwood R, Hollamby G, Rathjen T, Gororo N (eds) Proceedings of 10th Australian wheat breeding assembly, Wheat Breeding Society of Australia, Mildura, VIC, 16–21 September 2001, pp 26–31
Gras PW, Anderssen RS, Keentock M, Bekes F, Appels R (2001) Gluten protein functionality in wheat flour processing: a review. Aust J Agric Res 52:1311–1323
Gupta RB, Shepherd KW (1990) Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. I. Variation and genetic control of the subunits in hexaploid wheats. Theor Appl Genet 80:65–74
Gupta RB, Singh NK, Shepherd KW (1989) The cumulative effects of allelic variation in LMW and HMW glutenin subunits on physical dough properties in the progeny of two bread wheats. Theor Appl Genet 77:57–62
Gupta RB, Bekes F, Wrigley CW (1991) Prediction of physical dough properties from glutenin subunit composition in bread wheats. Cereal Chem 68:328–333
Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their application in wheat breeding. Plant Breed 118:369–390
He ZH, Liu L, Liu JJ, Xia XC, Peña RJ (2005) Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chem 82:345–350
He XY, He ZH, Zhang LP, Sun DJ, Morris CF, Fuerest EP, Xia XC (2007) Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theor Appl Genet 115:47–58
Huang XQ, Cloutier S (2008) Molecular characterization and genomic organization of low molecular weight glutenin subunit genes at the Glu-3 loci in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 116:953–966
Ikeda TM, Nagamine T, Fukuoka H, Yano H (2002) Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor Appl Genet 104:680–687
Ikeda TM, Araki E, Fujita Y, Yano H (2006) Characterization of low-molecular-weight glutenin subunit genes and their protein products in common wheats. Theor Appl Genet 112:327–334
Jackson EA, Morel MH, Sontag-Strohm T, Branlard G, Metakovsky EV, Redaelli R (1996) Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat (Triticum aestivum L.). J Genet Breed 50:321–336
Jiang CX, Pei YH, Zhang YZ, Li XH, Yao DN, Yan YM, Ma WJ, Hsam SLK, Zeller FJ (2008) Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi. Hereditas 145:92–98
Lei ZS, Gale KR, He ZH, Gianibeli C, Larroque O, Xia XC, Butow BJ, Ma W (2006) Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. J Cereal Sci 43:94–101
Lew EJL, Kuzmicky DD, Kasarda DD (1992) Characterization of low-molecular-weight glutenin subunits by reversed-phase high-performance liquid chromatography, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and N-terminal amino acid sequencing. Cereal Chem 69:508–515
Lindsay MP, Skerritt JH (1999) The glutenin macropolymer of wheat flour doughs: structure–function perspectives. Trends Food Sci Technol 10:247–253
Long H, Wei YM, Yan ZH, Baum B, Nevo E, Zhen YL (2005) Classification of wheat low-molecular-weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers. Theor Appl Genet 111:1251–1259
Luo C, Griffin WB, Branlard G, McNeil DL (2001) Comparison of low- and high- molecular-weight wheat glutenin allele effects on flour quality. Theor Appl Genet 102:1088–1098
Ma W, Zhang W, Gale KR (2003) Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica 134:51–60
Margiotta B, Colaprico G, D’Ovidio R, Lafiandra D (1993) Characterization of high Mr subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoretic separation and restriction fragment length polymorphism (RFLP) analyses of their encoding genes. J Cereal Sci 17:221–236
Maruyama-Funatsuki W, Takata K, Funatsuki H, Tabiki T, Ito M, Nishio Z, Kato A, Saito K, Yahata E, Saruyama H, Yamauchi H (2005) Identification and characterization of a novel LMW-s glutenin gene of a Canadian Western extra-strong wheat. J Cereal Sci 41:47–57
Masci S, D’Ovidio R, Lafiandra D, Kasarda DD (1998) Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer. Plant Physiol 118:1147–1158
Masci S, Rovelli L, Kasarda DD, Vensel WH, Lafiandra D (2002) Characterisation and chromosomal localization of C-type low-molecular-weight glutenin subunits in the bread wheat cultivar Chinese Spring. Theor Appl Genet 104:422–428
Metakovsky EV, Wrigley CW, Bekes F, Gupta RB (1990) Gluten polypeptides as useful genetic markers of dough quality in Australian wheats. Aust J Agric Res 41:289–306
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Corfield KG (1979) Subunit composition of wheat glutenin proteins, isolated by gel filtration in a dissociating medium. Planta 145:83–88
Shewry PR, Halford NG (2002) Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot 53:947–958
Singh NK, Shepherd KW (1988) Linkage mapping of genes controlling endosperm storage proteins in wheat. 1. Genes on the short arms of group-1 chromosomes. Theor Appl Genet 75:628–641
Van Campenhout S, Stappen JV, Sagi L, Volckaert G (1995) Locus-specific primers for LMW glutenin genes on each of the group 1 chromosomes of hexaploid wheat. Theor Appl Genet 91:313–319
Zhang W, Gianibelli MC, Ma W, Rampling L, Gale KR (2003) Identification of SNPs and development of AS-PCR markers for γ-gliadin alleles in Triticum aestivum. Theor Appl Genet 107:130–138
Zhang W, Gianibelli MC, Rampling L, Gale KR (2004) Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum L.). Theor Appl Genet 108:1409–1419
Zhao XL, Xia XC, He ZH, Gale KR, Lei ZS, Appels R, Ma WJ (2006) Characterization of three low-molecular-weight Glu-D3 subunit genes in common wheat. Theor Appl Genet 113:1247–1259
Zhao XL, Xia XC, He ZH, Lei ZS, Appels R, Yang Y, Sun QX, Ma W (2007a) Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat. Theor Appl Genet 114:451–460
Zhao XL, Ma W, Gale KR, Lei ZS, He ZH, Sun QX, Xia XC (2007b) Identification of SNPs and development functional markers for LMW-GS genes at Glu-D3 and Glu-B3 loci in bread wheat (Triticum aestivum L.). Mol Breed 20:223–231
Acknowledgments
The authors are very grateful to Prof. Robert McIntosh, University of Sydney, for reviewing this manuscript. Aroona and its NILs were kindly provided by Dr. Marie Appelbee and Prof. Ken Shepherd, SARDI Grain Quality Research Laboratory, Adelaide, South Australia, Cheyenne was provided by Dr. Baoyun Li, China Agricultural University, Beijing, and Chinese Spring and nulli-tetrasomic lines N1A-T1D, N1B-T1A, N1B-T1D and N1D-T1B were provided by Prof. Robert McIntosh. The study was supported by the National Science Foundation of China (30671296 and 30830072), National Basic Research Program (2009CB118300) and National 863 Programs (2006AA10Z1A7 and 2006AA100102).
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Communicated by J. W. Snape.
L. H. Wang and X. L. Zhao contributed equally to this study.
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Wang, L.H., Zhao, X.L., He, Z.H. et al. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor Appl Genet 118, 525–539 (2009). https://doi.org/10.1007/s00122-008-0918-9
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DOI: https://doi.org/10.1007/s00122-008-0918-9