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Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety

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Abstract

Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour.

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Acknowledgments

This work was supported by grants from the National Academy of Agricultural Science (PJ010116), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea

    Jong-Yeol Lee, Hye-Rang Beom, Sun-Hyung Lim, Ung-Han Yoon & Young-Mi Kim

  2. USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA, 94710, USA

    Susan B. Altenbach

  3. Department of Bioresource Sciences, Andong National University, Andong, 760-749, Korea

    Yeong-Tae Kim

  4. National Institute of Crop Science, RDA, Jeonju, 565-851, Korea

    Chon-Sik Kang

  5. Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Korea

    Ravi Gupta & Sun-Tae Kim

  6. Department of Agronomy, Chungnam National University, Daejeon, 357-764, Korea

    Sang-Nag Ahn

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  1. Jong-Yeol Lee
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Lee, JY., Beom, HR., Altenbach, S.B. et al. Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Funct Integr Genomics 16, 269–279 (2016). https://doi.org/10.1007/s10142-016-0482-3

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  • DOI: https://doi.org/10.1007/s10142-016-0482-3

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