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Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L)

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Abstract

PCR was used to amplify low-molecular-weight (LMW) glutenin genes from the Glu-A3 loci of hexaploid wheat cultivars containing different Glu-A3 alleles. The complete coding sequence of one LMW glutenin gene was obtained for each of the seven alleles Glu-A3a to Glu-A3g. Chromosome assignment of PCR products using Chinese Spring nulli-tetrasomic lines confirmed the amplified products were from chromosome 1A. All sequences were classified as LMW-i-type genes based on the presence of an N-terminal isoleucine residue and eight cysteine residues located within the C-terminal domain of the predicted, mature amino acid sequence. All genes contained a single uninterrupted open reading frame, including the sequence from the Glu-A3e allele, for which no protein product has been identified. Comparison of LMW glutenin gene sequences obtained from different alleles showed a wide range of sequence identity between the genes, with between 1 and 37 single nucleotide polymorphisms and between one and five insertion/deletion events between genes from different alleles. Allele-specific PCR markers were designed based on the DNA polymorphisms identified between the LMW glutenin genes, and these markers were validated against a panel of cultivars containing different Glu-A3 alleles. This collection of markers represents a valuable resource for use in marker-assisted breeding to select for specific alleles of this important quality-determining locus in bread wheat.

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Acknowledgements

This work was supported by Graingene, a research consortium of CSIRO Plant Industry, AWB Ltd and the Grains Research and Development Corporation.

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  1. W. Zhang

    Present address: Department of Agronomy and Range Science, University of California, Davis, CA 95616, USA

Authors and Affiliations

  1. Commonwealth Scientific and Industrial Research Organisation, Plant Industry, GPO Box 1600, ACT 2601, Canberra, Australia

    W. Zhang, M. C. Gianibelli, L. R. Rampling & K. R. Gale

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  1. W. Zhang
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  2. M. C. Gianibelli
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  4. K. R. Gale
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Correspondence to K. R. Gale.

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Communicated by P. Langridge

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Zhang, W., Gianibelli, M.C., Rampling, L.R. et al. Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L). Theor Appl Genet 108, 1409–1419 (2004). https://doi.org/10.1007/s00122-003-1558-8

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