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Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding

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Abstract

Bread wheat quality is mainly correlated with high molecular weight glutenin subunits (HMW-GS) of endosperm. The number of HMW-GS alleles with good processing quality is limited in bread wheat cultivars, while there are plenty of HMW-GS alleles in wheat-related grasses to exploit. We report here on the cloning and characterization of HMW-GS alleles from the decaploid Agropyron elongatum. Eleven novel HMW-GS alleles were cloned from the grass. Of them, five are x-type and six y-type glutenin subunit genes. Three alleles Aex4, Aey7, and Aey9 showed high similarity with another three alleles from the diploid Lophopyrum elongatum, which provided direct evidence for the Ee genome origination of A. elongatum. It was noted that C-terminal regions of three alleles of the y-type genes Aey8, Aey9, and Aey10 showed more similarity with x-type genes than with other y-type genes. This demonstrates that there is a kind of intermediate state that appeared in the divergence between x- and y-type genes in the HMW-GS evolution. One x-type subunit, Aex4, with an additional cysteine residue, was speculated to be correlated with the good processing quality of wheat introgression lines. Aey4 was deduced to be a chimeric gene from the recombination between another two genes. How the HMW-GS genes of A. elongatum may contribute to the improvement of wheat processing quality are discussed.

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References

  • Anderson OD, Greene FC (1989) The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid wheat. Theor Appl Genet 77:689–700

    Article  CAS  Google Scholar 

  • Anderson OD, Greene FC (1997) The α-gliadin gene family. II. DNA and protein sequence variation, subfamily structure, and origins of pseudogenes. Theor Appl Genet 95:59–65

    Article  CAS  Google Scholar 

  • Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462

    Article  PubMed  CAS  Google Scholar 

  • Arguello JR, Chen Y, Yang S, Wang W, Long M (2006) Origination of an X-linked testes chimeric gene by illegitimate recombination in Drosophila. PLoS Genet 2(5):e77

    Article  PubMed  CAS  Google Scholar 

  • Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354

    CAS  Google Scholar 

  • De Bustos A, Rubio P, Jouve N (2000) Molecular characterization of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat. Theor Appl Genet 100:1085–1094

    Article  Google Scholar 

  • De Bustos A, Rubio P, Jouve N (2001) Characterisation of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat. Theor Appl Genet 103:733–742

    Article  Google Scholar 

  • Feng DS, Xia GM, Zhao SY, Chen FG (2004a) Two quality-associated HMW glutenin subunits in a somatic hybrid line between Triticum aestivum and Agropyron elongatum. Theor Appl Genet 110:136–144

    Article  PubMed  CAS  Google Scholar 

  • Feng DS, Chen FG, Zhao SY, Xia GM (2004b) High-molecular-weight glutenin subunit genes in decaploid Agropyron elongatum. Acta Bot Sin 46:489–496

    CAS  Google Scholar 

  • Feng DS, Chen FG, Zhao SY, Xia GM (2004c) Study on a novel HMW glutenin subunit coding region from Agropyron elongatum (in Chinese with English Abstract). Acta Bot Boreal Occident Sin 24:237–242

    CAS  Google Scholar 

  • Forde J, Malpica J-M, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832

    Article  PubMed  CAS  Google Scholar 

  • Guo ZF, Yan ZH, Wang JR, Wei YM, Zheng YL (2005) Characterization of HMW prolamines and their coding sequences from Crithopsis delileana. Hereditas 142:56–64

    Article  PubMed  Google Scholar 

  • Gupta RB, MacRitchie F (1994) Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1, of common wheats. II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci 19:19–29

    Article  CAS  Google Scholar 

  • Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126

    Article  CAS  Google Scholar 

  • Halford NG, Forde J, Shewry PR, Kreis M (1989) Functional analysis of the upstream regions of a silent and an expressed member of a family of wheat seed protein genes in transgenic tobacco. Plant Sci 62:207–216

    Article  CAS  Google Scholar 

  • Halford NG, Field JM, Blair H, Urwin P, Moore K, Robert L, Thompson R, Flavell RB, Tatham AS, Shewry PR (1992) Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83:373–378

    Article  CAS  Google Scholar 

  • Harberd NP, Bartels D, Thompson RD (1986) DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem Genet 24:579–596

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  PubMed  CAS  Google Scholar 

  • Lafiandra D, D’Ovidio R, Porceddu E, Margiotta B, Colaprico G (1993) New data supporting high Mr glutenin subunit 5 as the determinant of quality differences among the pairs 5 + 10 vs. 2 + 12. J Cereal Sci 18:197–205

    Article  CAS  Google Scholar 

  • Lawrence GJ, Shepherd KW (1980) Variation in glutenin protein subunits of wheat. Aust J Biol Sci 33:221–233

    Article  CAS  Google Scholar 

  • Lawrence GJ, Shepherd KW (1981) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31

    CAS  Google Scholar 

  • Liu Z, Yan Z, Wang Y, Liu K, Zheng Y, Wang D (2003) Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theor Appl Genet 106:1368–1378

    PubMed  CAS  Google Scholar 

  • Liu H, Shi L, Zhao JS, Xia GM (2006) Genetic characteristic of high molecular weight glutenin subunits in somatic hybrid wheat lines––potential application to wheat breeding. J Agric Food Chem 54:5007–5013

    Article  CAS  Google Scholar 

  • Liu S, Zhao S, Chen F, Xia G (2007) Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum. BMC Evol Biol 7:76

    Article  PubMed  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120

    Article  CAS  Google Scholar 

  • Payne PI, Holt LM, Worland AJ, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138

    Article  CAS  Google Scholar 

  • Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the breadmaking quality of British grown wheat varieties. J Sci Food Agric 40:51–65

    Article  CAS  Google Scholar 

  • Payne PI, Holt LM, Krattiger AF, Carrillo JM (1988) Relationship between seed quality characteristics and HMW glutenin subunit composition determined using wheats grown in Spain. J Cereal Sci 7:229–235

    Article  CAS  Google Scholar 

  • Rafalski JA (1986) Structure of wheat gamma-gliadin genes. Gene 43:221–229

    Article  PubMed  CAS  Google Scholar 

  • Reddy P, Appels R (1993) Analysis of a genomic DNA segment carrying the wheat high-molecular-weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker. Theor Appl Genet 85:616–624

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Shewry PR, Tatham AS (1990) The prolamin storage proteins of cereal seeds: structure and evolution. Biochem J 267:1–12

    PubMed  CAS  Google Scholar 

  • Shewry PR, Napier JA, Tatham AS (1995) Seed storage proteins: structure and biosynthesis. Plant Cell 7:945–956

    Article  PubMed  CAS  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003a) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res 45:219–302

    PubMed  CAS  Google Scholar 

  • Shewry PR, Halford NG, Lafiandra D (2003b) Genetics of wheat gluten proteins. Adv Genet 49:111–184

    PubMed  CAS  Google Scholar 

  • Sugiyama T, Rafalski A, Peterson D, Söll D (1985) A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737

    Article  PubMed  CAS  Google Scholar 

  • Sun X, Hu SL, Liu X, Qian WQ, Hao ST, Zhang AM, Wang DW (2006) Characterization of the HMW glutenin subunits from Aegilops searsii and identification of a novel variant HMW glutenin subunit. Theor Appl Genet 113:631–641

    Article  PubMed  CAS  Google Scholar 

  • Thompson RD, Bartels D, Harberd NP (1985) Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13:6833–6846

    Article  PubMed  CAS  Google Scholar 

  • Wang W, Brunet FG, Nevo E, Long M (2002) Origin of Sphinx, a young chimeric RNA gene in Drosophila melanogaster. Proc Natl Acad Sci USA 99:4448–4453

    Article  PubMed  CAS  Google Scholar 

  • Wang JR, Yan ZH, Wei YM, Zheng YL (2004) A novel high-molecular-weight glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski. J Cereal Sci 40:289–294

    Article  CAS  Google Scholar 

  • Wang JR, Yan ZH, Wei YM, Zheng YL (2006) Characterization of high molecular weight glutenin subunit genes from Elytrigia elongata. Plant Breed 125:89–95

    Article  CAS  Google Scholar 

  • Wan Y, Wang D, Shewry PR, Halford NG (2002) Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet 104:828–839

    Article  PubMed  CAS  Google Scholar 

  • Xia GM, Xiang FN, Zhou AF, Wang H, Chen HM (2003) Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107:299–305

    Article  PubMed  CAS  Google Scholar 

  • Yan ZH, Wei YM, Wang JR, Liu DC, Dai SF, Zheng YL (2006) Characterization of two HMW glutenin subunit genes from Taenitherum Nevski. Genetica 127:267–276

    Article  PubMed  CAS  Google Scholar 

  • Zhang XY, Dong YS, Wang RRC (1996) Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum × Thinopyrum ponticum by in situ hybridization, isozyme analysis and RAPD. Genome 39:1062–1071

    Article  CAS  PubMed  Google Scholar 

  • Zhao TJ, Quan TY, Xia GM, Chen HM (2003) Glutenin and SDS sedimentation analysis of the F5 somatic hybrids between Triticum aestivum L. and Agropyron elongatum (in Chinese with English Abstract). J Shandong Univ (Nat Sci) 38(3):112–116

    CAS  Google Scholar 

  • Zhou HP, Li B, Li ZS (1995) The study of breeding blue-grain gene translocation of wheat (in Chinese with English Abstract). Acta Bot Boreal Occident Sin 15:125–128

    Google Scholar 

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Acknowledgments

This work was supported by Projects of National 863 High Technology Research and Development (Project no. 2006AA100102), National Basic Research Program of China (Project no. 2006CB100100), and Key and Doctor Position of ministry of education.

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  1. School of Life Sciences, Shandong University, 250100, Jinan, People’s Republic of China

    Shuwei Liu, Xin Gao & Guangmin Xia

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  1. Shuwei Liu
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  2. Xin Gao
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  3. Guangmin Xia
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Correspondence to Guangmin Xia.

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Communicated by M. Morell.

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Liu, S., Gao, X. & Xia, G. Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding. Theor Appl Genet 116, 325–334 (2008). https://doi.org/10.1007/s00122-007-0669-z

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  • DOI: https://doi.org/10.1007/s00122-007-0669-z

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