Abstract
Restriction enzyme digests of DNA from nullisomic-tetrasomic and intervarietal chromosome substitution lines of wheat were probed with a high molecular weight (HMW) glutenin cDNA. Three restriction endonucleases were used to investigate restriction-fragment differences among five wheat varieties. The results suggest that the hybridizing fragments contain single gene copies and permit the identification of the subunit encoded by each gene. Restriction-fragment variation associated with previously established allelic differences between varieties was observed. Also, there is a clear relationship between the electrophoretic mobility of a HMW subunit and the length of the central repetitive section of the gene encoding it. These results are discussed with reference to the evolution of the HMW glutenin gene family and the uses of restriction-fragment variation in plant breeding and genetics.
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Baulcombe, D., Lazarus, C., and Martienssen, R. (1984). Gibberellins and gene control in cereal aleurone cells. J. Embryol. Exp. Morphol. 83 (Suppl.):119.
Burr, B., Evola, S. V., Burr, F. A., and Beckmann, J. S. (1983). The application of restriction fragment length polymorphism to plant breeding. In Setlow, J., and Hollaender, A. (eds.), Genetic Engineering: Principles and Methods, Vol 5 Plenum Press, New York, pp. 45–60.
Feldman, M. (1976). Wheats. In Simmonds, N. W. (ed.), Evolution of Crop Plants Longman, London, pp. 120–128.
Flavell, R. B. (1980). The molecular characterization and organization of plant chromosomal DNA sequences. Annu. Rev. Plant. Physiol. 31569.
Forde, J., Malpica, J.-M., Halford, N. G., Shewry, P. R., Anderson, O. D., Greene, F. C., and Miflin, B. J. (1985). The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L). Nucleic Acids Res. 136817.
Harberd, N. P., Bartels, D., and Thompson, R. D. (1985). Analysis of the gliadin multigene loci in bread wheat using nullisomic-tetrasomic lines. Mol. Gen. Genet. 198234.
Johns, M. A., Strommer, J. N., and Freeling, M. (1983). Exceptionally high levels of restriction site polymorphism in DNA near the maize Adh1 gene. Genetics 105733.
Kimber, G. (1974). A reassessment of the origin of polyploid wheats. Genetics 78487.
Langley, C. H., Montgomery, E. A., and Quattlebaum, W. F. (1982). Restriction map variation in the Adh region of Drosophila. Proc. Natl. Acad. Sci. USA 795631.
Law, C. N., and Worland, A. J. (1973). Aneuploidy in wheat and its uses in genetic analysis. Annu. Rep. Plant Breed. Inst., 1972, pp. 25–65.
Lazarus, C. M., Baulcombe, D. C., and Martienssen, R. A. (1985). α-Amylase genes of wheat are two multigene families which are differentially expressed. Plant Mol. Biol. 513.
Payne, P. I., Law, C. N., and Mudd, E. E. (1980). Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet. 58113.
Payne, P. I., Corfield, K. G., Holt, L. M., and Blackman, J. A. (1981a). Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread making quality in progenies of six crosses of bread wheat. J. Sci. Food Agr. 3251.
Payne, P. I., Holt, L. M., and Law, C. N. (1981b). Structural and genetical studies on the high-molecular-weight subunits of wheat gluten 1. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor. Appl. Genet. 60229.
Payne, P. I., Holt, L. M., Worland, A. J., and Law, C. N. (1982). Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theor. Appl. Genet. 63129.
Payne, P. I., Holt, L. M., Thompson, R. D., Bartels, D., Harberd, N. P., Harris, P. A., and Law, C. N. (1983). The high-molecular-weight subunits of glutenin: Classical genetics, molecular genetics and the relationship to bread-making quality. In Proc. 6th Int. Wheat Genet. Symp., Kyoto, Japan, pp. 827–834.
Payne, P. I., Holt, L. M., Jackson, E. A., and Law, C. N. (1984). Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Phil. Trans. R. Soc. Lond. B 304359.
Payne, P. I., Holt, L. M., and Jackson, E. A. (1985). Genetical analysis of wheat endosperm storage proteins. In Graveland, A., and Moonen, J. H. E. (eds.), Gluten Proteins TNO, Holland, pp. 111–119.
Riley, R. (1965). Cytogenetics and the evolution of wheat. In Hutchinson, J. B. (ed.), Essays on Crop Plant Evolution Cambridge University Press, Cambridge, pp. 103–122.
Sears, E. R. (1953). Nullisomic analysis in common wheat. Am. Nat. 87245.
Sears, E. R. (1954). The aneuploids of common wheat. Mont. Agr. Exp. Sta. Bull. 5721.
Sears, E. R. (1966). Nullisomic-tetrasomic combinations in hexaploid wheat. In Chromosome Manipulations and Plant Genetics, Heredity Vol. 20 Suppl. Oliver and Boyd, Edinburgh, pp. 29–45.
Shewry, P. R., and Miflin, M. J. (1985). Seed storage proteins of economically important cereals. In Advances in Cereal Science and Technology, Vol. VII American Association of Cereal Chemists, St. Paul, Minn., pp. 1–83.
Shewry, P. R., Field, J. M., Faulks, A. J., Parmar, S., Miflin, B. J., Dietler, M. D., Lew, E. J-E., and Kasarda, D. D. (1984). The purification and N-terminal amino acid sequence analysis of the high-molecular-weight gluten polypeptides of wheat. Biochim. Biophys. Acta 78823.
Sugiyama, T., Rafalski, A., Peterson, D., and Soll, D. (1985). A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res. 138729.
Thompson, R. D., Bartels, D., Harberd, N. P., and Flavell, R. B. (1983). Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones. Theor. Appl. Genet. 6787.
Thompson, R. D., Bartels, D., and Harberd, N. P. (1985). Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW glutenin subunit. Nucleic Acids Res. 136883.
Wyman, A. R., and White, R. (1980). A high polymorphic locus in human DNA. Proc. Natl. Acad. Sci. USA 776754.
Zachar, Z., and Bingham, P. M. (1982). Regulation of white locus expression: The structure of mutant alleles at the white locus of Drosophila melanogaster. Cell 30529.
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N.P.H. was supported by a MRC Training Fellowship in Recombinant DNA Technology and a grant from the Perry Foundation. D.B. is supported by EEC Contract GBI-4-027-UK.
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Harberd, N.P., Bartels, D. & Thompson, R.D. DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem Genet 24, 579–596 (1986). https://doi.org/10.1007/BF00504336
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DOI: https://doi.org/10.1007/BF00504336