Summary
A gene encoding the high-molecular-weight (HMW) subunit of glutenin 1Ax1 was isolated from bread wheat cv Hope. Comparison of the deduced amino acid sequence with that previously reported for an allelic subunit, 1Ax2*, showed only minor differences, which were consistent with both subunits being associated with good bread-making quality. Quantitative analyses of total protein extracts from 22 cultivars of bread wheat showed that the presence of either subunit 1Ax1 or 1Ax2*, when compared with a null allele, resulted in an increase in the proportion of HMW subunit protein from ca. 8 to 10% of the total. It is suggested that this quantitative increase in HMW subunit protein may account for the association of 1Ax subunits with good quality.
Similar content being viewed by others
References
Anderson OD, Greene FC (1989) The characterisation and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of hexaploid bread wheat. Theor Appl Genet 77:689–700
Autran J-C (1990) Variabilité génétique et agronomique de la composition quantitative en HMW-gluténines et en protéines agregatives des blés. Proc INRA Coll, 4 Dec 1990, Paris, pp 3–29
Bunce N, White RP, Shewry PR (1985) Variation in estimates of molecular weights of cereal prolamins by SDS-PAGE. J Cereal Sci 3:131–142
Chou PY, Fasman GD (1978) Empirical predictions of protein conformations. Annu Rev Biochem 47:251–276
Datta SK, Peterhans A, Datta K, Potrykus I (1990) Genetically engineered fertile Indica rice recovered from protoplasts. BioTechnology 8:741–745
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
Field JM, Shewry PR, Miflin BJ (1983) Solubilization and characterization of wheat gluten proteins; correlations between the amount of aggregated proteins and baking quality. J Sci Food Agric 34:370–377
Flavell RB, Goldsbrough AP, Robert LS, Schnick D, Thompson RD (1989) Genetic variation in wheat HMW glutenin subunits and the molecular basis of breadmaking quality. BioTechnology 7:1281–1285
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Inheritance and expression of chimaeric genes in the progeny of transgenic maize plants. BioTechnology 8:833–839
Genstat 5 Committee (1990) Genstat 5, Release 2 reference manual supplement. Statistics Department, Rothamsted Experimental Station, Harpenden, UK
Gordon-Kamm WJ, Spencer TM, Mangano ML, Adam TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lamaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2:603–618
Harberd NP, Bartels D, Thompson RD (1986) DNA restriction fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem Genet 24:579–596
Lawrence GJ, Shepherd KW (1981) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Marchylo BA, Kruger JE, Hatcher DW (1989) Quantitative reversed-phase high-performance liquid Chromatographie analysis of wheat storage proteins as a potential quality prediction tool. J Cereal Sci 9:113–130
Moonen JHE, Scheepstra A, Graveland A (1983) The positive effects of the high molecular weight subunits 3+10 and 2* of glutenin on the breadmaking quality of wheat cultivars. Euphytica 32:735–742
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Corfield KG, Blackman JA (1979) Identification of a high-molecular-weight subunit of glutenin whose presence correlates with breadmaking quality in wheats of related pedigree. Theor Appl Genet 55:153–159
Payne PI, Law CN, Mudd EE (1980) Control by homeologous group I chromosomes of the high molecular weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Payne PI, Corfield KG, Holt LM, Blackman JA (1981a) Correlations between the inheritance of certain high molecular weight subunits of glutenin and breadmaking quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60
Payne PI, Holt LM, Law CN (1981b) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Theor Appl Genet 60:229–236
Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the breadmaking quality of British-grown wheat varieties. J Sci Food Agric 40:51–65
Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988) Genetically transformed maize plants from protoplasts. Science 240:204–207
Seilmeier W, Belitz H-D, Wieser H (1991) Separation and quantitative determination of high-molecular-weight subunits of glutenin from different wheat varieties and genetic variants of the variety Sicco. Z Lebensm Unters Forsch 192:124–129
Shewry PR, Field JM, Faulks AJ, Parmar S, Miflin BJ, Dietler MD, Lew EJ-L, Kasarda DD (1984) Purification and N-terminal amino acid sequence analysis of high molecular weight (HMW) gluten polypeptides of wheat. Biochim Biophys Acta 788:23–34
Shewry PR, Halford NG, Tatham AS (1989) The high molecular weight subunits of wheat, barley and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. In: Miflin BJ (ed) Oxford Surveys of Plant Molecular and Cell Biology, vol 6. Oxford University Press, Oxford, pp 163–219
Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338:274–276
Sugiyama T, Rafalksi A, Peterson D, Soll D (1985) A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8739
Thompson RD, Bartels D, Harberd NP, Flavell RB (1983) Characterisation of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones. Theor Appl Genet 67:87–96
Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. BioTechnology 6:1072–1074
Vasil V, Redway F, Vasil IK (1990) Regeneration of plants from embryogenic suspension cultivar protoplasts of wheat (Triticum aestivum L.) BioTechnology 8:429–434
Author information
Authors and Affiliations
Additional information
Communicated by J. W. Snape
EMBL Data Library. Accession number: X61009
Rights and permissions
About this article
Cite this article
Halford, N.G., Field, J.M., Blair, H. et al. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theoret. Appl. Genetics 83, 373–378 (1992). https://doi.org/10.1007/BF00224285
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224285