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Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi

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Abstract

Suspension-derived protoplasts of Agropyron elongatum irradiated by ultra-violet light (UV) were fused with the suspension-derived protoplasts of Triticum astivum using PEG. Fertile intergeneric somatic hybrid plants were produced and various hybrid lines have been selected and propagated in successive generations. Their hybrid nature was confirmed by analysis of profiles of isozymes, RAPDs, and 5S rDNA spacer sequences, and via GISH analysis. By the procedure described, the phenotype and chromosome number of wheat could be maintained besides transfer of a few chromosomes and chromosomal fragments from the donor A. elongatum. The results above indicated that highly asymmetric fertile hybrid plants and hybrid progenies of wheat were produced via somatic hybridization.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China, No.30070397, the Trans-century Training Programme Foundation for Talents by the Ministry of Education in China and National 863 High Technology Research and Development Project No. 2001AA241032. We are grateful to Dr. Robert Koebner (John Innes Centre, UK) for language correction.

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Authors and Affiliations

  1. School of Life Sciences, Shandong University, Jinan 250100, P.R. China

    Guangmin Xia, Fengning Xiang, Aifen Zhou, Huai Wang & Huimin Chen

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  1. Guangmin Xia
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  2. Fengning Xiang
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  3. Aifen Zhou
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  4. Huai Wang
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  5. Huimin Chen
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Correspondence to Guangmin Xia.

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Communicated by K. Glimelius

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Xia, G., Xiang, F., Zhou, A. et al. Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107, 299–305 (2003). https://doi.org/10.1007/s00122-003-1247-7

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  • DOI: https://doi.org/10.1007/s00122-003-1247-7

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