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Molecular mapping of kernel shattering and its association with Fusarium head blight resistance in a Sumai3 derived population

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Abstract

Kernel shattering (KS) can cause severe grain yield loss in wheat (Triticum aestivum L.). The introduction of genotypes with Fusarium head blight (FHB) resistance has elevated the KS importance. ‘Sumai3,’ the most commonly used FHB-resistant germplasm worldwide, is reported to be KS susceptible. The objectives of this study were to detect quantitative trait loci (QTLs) for KS and to determine the relationship between KS and FHB. A recombinant inbred line population derived from a cross between Sumai3 and ‘Stoa’ was evaluated for KS in five environments and FHB in two field trials, separately. Four genomic regions on chromosomes 2B, 3B, and 7A were associated with KS. Of them, two major KS QTLs were detected consistently over three environments and each located proximal to the centromere on chromosomes 3B and 7A. The resistant alleles at these two QTLs together can reduce KS by 66.1% relative to the reciprocal alleles and by 41.1% compared to the population mean. The field FHB data revealed four QTLs on chromosomes 2B, 3B, and 7A. Three of these FHB QTLs coincided with and/or linked to the KS QTLs with opposite allele effects in the corresponding genomic regions, which may explain the negative correlation (r = −0.29 and P < 0.01) between the KS and FHB infection found in this study. The results in this study indicate that KS and FHB in Sumai3 are, in part, inherited dependently. However, the correlation between KS and FHB is not strong, and the major FHB resistance QTL on chromosome arm 3BS was not linked to any KS QTL. Our results showed that pyramiding of the two major KS-resistant alleles and the unlinked major FHB-resistant allele could produce lines with both low values of KS and FHB infection.

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Acknowledgments

The authors would like to thank Dr. S. Kianian and his laboratory group at the NDSU Plant Sciences Department to allow us to conduct part of this work in the wheat enhancement lab. Special thanks go Dr. J. Anderson at the U of MN, MN for the help regarding the markers. Thanks also to B. Schatz (NDSU Research and Extension Center, Carrington, ND, USA) for his help to conduct this research at Carrington Research and Extension Center, ND. We also thank Dr. J. Faris (USDA-ARS, Fargo, ND); and Drs. R. D. Horsley, D. W. Meyer, P. K. Singh, and X. Cai at the Plant Sciences Department (North Dakota State University, USA) for their inputs and reviewing the manuscript. Deep appreciation goes to the Hard Red Spring Wheat Breeding program personnel for their help to accomplish this work. This material is based upon work supported partly by the US Department of Agriculture, under Agreement No. 59-0790-4-100. This is a cooperative project with the US Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture.

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  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    Guorong Zhang & Mohamed Mergoum

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  1. Guorong Zhang
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Correspondence to Mohamed Mergoum.

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Communicated by G. Wenzel.

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Zhang, G., Mergoum, M. Molecular mapping of kernel shattering and its association with Fusarium head blight resistance in a Sumai3 derived population. Theor Appl Genet 115, 757–766 (2007). https://doi.org/10.1007/s00122-007-0606-1

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